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484R-1

Sigma-Aldrich

PRAME (EP461) Rabbit Monoclonal Primary Antibody

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Synonym(s):
Preferentially-expressed Antigen in Melanoma

biological source

rabbit

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

EP461, monoclonal

description

For In Vitro Diagnostic Use in Select Regions

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (484R-14)
vial of 0.1 mL concentrate Research Use Only (484R-14-RUO)
vial of 0.5 mL concentrate (484R-15)
vial of 1.0 mL concentrate (484R-16)
vial of 1.0 mL concentrate Research Use Only (484R-16-RUO)
vial of 1.0 mL pre-dilute Research Use Only (484R-17-RUO)
vial of 1.0 mL pre-dilute ready-to-use (484R-17)
vial of 25.0 mL pre-dilute ready-to-use Research Use Only (484R-10-RUO)
vial of 25.0 mL pre-dilute ready-to-use (484R-10)
vial of 7.0 mL pre-dilute ready-to-use (484R-18)
vial of 7.0 mL pre-dilute ready-to-use Research Use Only (484R-18-RUO)

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:50 (concentrated)

isotype

IgG

control

melanoma

shipped in

wet ice

storage temp.

2-8°C

visualization

nuclear

General description

PRAME (PReferentially-expressed Antigen in MElanoma) is a gene encoded on the 22q11-22 chromosomal sequence and encodes a 509 amino acid residue protein.1 PRAME is a melanoma antigen that is preferentially expressed in tumors and is recognized by cytotoxic T lymphocytes.2,3 PRAME can be used to distinguish between malignant melanoma cells and nevus cells,4 and therefore may be useful for diagnostic purposes to support a suspected case of melanoma. PRAME is considered a cancer-testis antigen (CTA)5 and is not strongly expressed in most other normal tissues. PRAME is positively expressed in about half of uveal melanomas,6 and the majority of mucosal melanomas.7

Quality

  • United States - IVD
  • Canada - RUO
  • European Union - IVD
  • Japan - RUO

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and < 0.1% Sodium Azide.

Preparation Note

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Other Notes

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Frances Wadelin et al.
Molecular cancer, 9, 226-226 (2010-08-31)
PRAME/MAPE/OIP4 is a germinal tissue-specific gene that is also expressed at high levels in haematological malignancies and solid tumours. The physiological functions of PRAME in normal and tumour cells are unknown, although a role in the regulation of retinoic acid
Aimi Toyama et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 32(12), 1727-1733 (2019-08-04)
Mucosal melanomas are rare, and less is known about the biomarkers of this subtype in comparison to cutaneous or uveal melanomas. Preferentially expressed antigen in melanoma (PRAME) has been studied as a tool for prognostication of uveal melanomas, and immunotherapy
Gülçin Gezgin et al.
JAMA ophthalmology, 135(6), 541-549 (2017-04-28)
Uveal melanoma (UM) is an intraocular primary malignant neoplasm that often gives rise to metastatic disease for which there are no effective therapies. A substantial proportion of UMs express the cancer-testis antigen PRAME (preferentially expressed antigen in melanoma), which can
Cecilia Lezcano et al.
The American journal of surgical pathology, 42(11), 1456-1465 (2018-07-26)
PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen that was isolated by autologous T cells in a melanoma patient. While frequent PRAME mRNA expression is well documented in cutaneous and ocular melanomas, little is known about PRAME protein
H Ikeda et al.
Immunity, 6(2), 199-208 (1997-02-01)
Melanoma lines MEL.A and MEL.B were derived from metastases removed from patient LB33 in 1988 and 1993, respectively. The MEL.A cells express several antigens recognized by autologous cytolytic T lymphocytes (CTL) on HLA class I molecules. The MEL.B cells have

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