484R-1

PRAME (EP461) Rabbit Monoclonal Primary Antibody

• Synonym(s): Preferentially-expressed Antigen in Melanoma
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 100; 500
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: EP461, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (484R-14); vial of 0.1 mL concentrate Research Use Only (484R-14-RUO); vial of 0.5 mL concentrate (484R-15); vial of 1.0 mL concentrate (484R-16); vial of 1.0 mL concentrate Research Use Only (484R-16-RUO); vial of 1.0 mL pre-dilute Research Use Only (484R-17-RUO); vial of 1.0 mL pre-dilute ready-to-use (484R-17); vial of 25.0 mL pre-dilute ready-to-use Research Use Only (484R-10-RUO); vial of 25.0 mL pre-dilute ready-to-use (484R-10); vial of 7.0 mL pre-dilute ready-to-use (484R-18); vial of 7.0 mL pre-dilute ready-to-use Research Use Only (484R-18-RUO)
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:50 (concentrated)
• isotype: IgG
• control: melanoma
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: nuclear

Description

General description

PRAME (PReferentially-expressed Antigen in MElanoma) is a gene encoded on the 22q11-22 chromosomal sequence and encodes a 509 amino acid residue protein.¹ PRAME is a melanoma antigen that is preferentially expressed in tumors and is recognized by cytotoxic T lymphocytes.^2,3 PRAME can be used to distinguish between malignant melanoma cells and nevus cells,⁴ and therefore may be useful for diagnostic purposes to support a suspected case of melanoma. PRAME is considered a cancer-testis antigen (CTA)⁵ and is not strongly expressed in most other normal tissues. PRAME is positively expressed in about half of uveal melanomas,⁶ and the majority of mucosal melanomas.⁷

Quality

• United States - IVD
• Canada - RUO
• European Union - IVD
• Japan - RUO

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and < 0.1% Sodium Azide.

Preparation Note

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Other Notes

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Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable