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59085

Sigma-Aldrich

3-Methylbutanol

BioUltra, for molecular biology, ≥99.0% (GC)

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Synonym(s):
3-Methyl-1-butanol, Isoamyl alcohol, Isopentyl alcohol
Linear Formula:
(CH3)2CHCH2CH2OH
CAS Number:
Molecular Weight:
88.15
Beilstein:
1718835
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

Assay

≥99.0% (GC)

form

liquid

technique(s)

DNA extraction: suitable
RNA extraction: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.01% free acid (as CH3COOH)

evapn. residue

≤0.003%

refractive index

n20/D 1.407

pH

5.6 (20 °C, 25 g/L)

density

0.809 g/mL at 20 °C (lit.)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤0.5 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.06

SMILES string

CC(C)CCO

InChI

1S/C5H12O/c1-5(2)3-4-6/h5-6H,3-4H2,1-2H3

InChI key

PHTQWCKDNZKARW-UHFFFAOYSA-N

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This Item
I9392AX1440M32658
3-Methylbutanol BioUltra, for molecular biology, ≥99.0% (GC)

Sigma-Aldrich

59085

3-Methylbutanol

Premium Grade
3-Methylbutanol BioReagent, for molecular biology, ≥98.5%

Sigma-Aldrich

I9392

3-Methylbutanol

-
Isoamyl Alcohol Meets ACS Specifications GR ACS

Supelco

AX1440

Isoamyl Alcohol

-
3-Methyl-1-butanol reagent grade, 98%

Sigma-Aldrich

M32658

3-Methyl-1-butanol

Essential Grade
assay

≥99.0% (GC)

assay

≥98.5%

assay

≥98.5% (corrected for water content, GC)

assay

98%

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

DNA extraction: suitable, RNA extraction: suitable

technique(s)

RNA extraction: suitable

technique(s)

mass spectrometry (MS): suitable

technique(s)

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.01% free acid (as CH3COOH)

impurities

-

impurities

≤0.002 meq/g Titrable acid, ≤0.1% Carbonyl compounds (as HCHO), ≤0.2% Acids and esters (as amyl acetate), ≤0.5% Water (H2O)

impurities

-

refractive index

n20/D 1.407

refractive index

-

refractive index

n20/D 1.406 (lit.)

refractive index

n20/D 1.406 (lit.)

Application

Isoamyl alcohol (3-methylbutanol) may be used as a protein denaturation component of various RNA and DNA extraction and purification protocols. Isoamyl alcohol is frequently used together with phenol and or chloroform in nucleic acid preparation protocols.

Other Notes

Reduces interface between 2 phases

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

110.3 °F - closed cup

Flash Point(C)

43.5 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Basic Methods in Molecular Biology (1986)
Mary J Dunlop et al.
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Nature communications, 3, 739-739 (2012-03-15)
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Malaria journal, 10, 28-28 (2011-02-10)
Anopheles gambiae sensu stricto is considered to be highly anthropophilic and volatiles of human origin provide essential cues during its host-seeking behaviour. A synthetic blend of three human-derived volatiles, ammonia, lactic acid and tetradecanoic acid, attracts A. gambiae. In addition
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Differences in the production of bacterial metabolites with potential impact on fermented sausage flavour were found in meat simulation medium when comparing different strains of Staphylococcus xylosus and Staphylococcus carnosus as starter cultures. Overall, higher levels of 3-methyl-1-butanol and acetoin

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