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A3641

Sigma-Aldrich

Apoferritin from equine spleen

0.2 μm filtered

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CAS Number:
MDL number:
NACRES:
NA.32

Quality Level

sterility

0.2 μm filtered

form

liquid

mol wt

major subunit ML 19,889
minor subunit MH 22,200
native ~481.2 kDa (24 subunits, approx. 20 kDa each)

color

clear to slightly hazy, solution

storage temp.

2-8°C

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This Item
F4503V8379A8706
vibrant-m

A3641

Apoferritin from equine spleen

vibrant-m

F4503

Ferritin from equine spleen

vibrant-m

V8379

Vitronectin from human plasma

vibrant-m

A8706

Avidin from egg white

mol wt

major subunit ML 19,889, native ~481.2 kDa (24 subunits, approx. 20 kDa each), minor subunit MH 22,200

mol wt

H subunit ~21 kDa, L subunit ~19 kDa

mol wt

A subunit 75 kDa, B subunit 65 kDa

mol wt

glycoprotein 66 kDa, subunit 16 kDa

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

200

sterility

0.2 μm filtered

sterility

sterile-filtered

sterility

-

sterility

-

color

clear to slightly hazy, solution

color

red to brown

color

-

color

-

Application

Apoferritin from equine spleen has been used:
  • to determine its partition coefficients in phase systems
  • in NaCl for iron loading of cultured cells
  • in in situ liquid scanning transmission electron microscope for imaging the interface of biology and nanotechnology

Biochem/physiol Actions

This protein shell of ferritin lacking iron is widely used for the calibration of gel filtration columns and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. It is very abundant in beta-cells of the pancreas where it acts as an anti-oxidant. When added to cultured endothelial cells apoferritin is taken up in a dose-responsive manner and appears to protect the cells from oxidant-mediated cytolysis.

Physical form

Solution in 0.135 M sodium chloride.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy
Evans JE, et al.
Micron (Oxford, England : 1993), 43(11), 1085-1090 (2012)
Ferritin-iron increases killing of Chinese hamster ovary cells by X-irradiation
Nelson JM and Stevens RG
Cell proliferation, 25(6), 579-585 (1992)
Jorge H Melillo et al.
Scientific reports, 12(1), 16512-16512 (2022-10-04)
Some of the best nucleating agents in nature are ice-nucleating proteins, which boost ice growth better than any other material. They can induce immersion freezing of supercooled water only a few degrees below 0 °C. An open question is whether this
G Balla et al.
The Journal of biological chemistry, 267(25), 18148-18153 (1992-09-05)
Phagocyte-mediated oxidant damage to vascular endothelium is likely involved in various vasculopathies including atherosclerosis and pulmonary leak syndromes such as adult respiratory distress syndrome. We have shown that heme, a hydrophobic iron chelate, is rapidly incorporated into endothelial cells where
Masahide Takehara et al.
Analytical biochemistry, 373(2), 322-329 (2007-12-07)
Two kinds of layer silicate powder, Micromica and chlorite, were used to aid protein crystallization by the addition to hanging drops. Using appropriate crystallization buffers, Micromica powder facilitated crystal growth speed for most proteins tested in this study. Furthermore, the

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