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Quality Level
sterility
0.2 μm filtered
form
liquid
mol wt
major subunit ML 19,889
minor subunit MH 22,200
native ~481.2 kDa (24 subunits, approx. 20 kDa each)
color
clear to slightly hazy, solution
storage temp.
2-8°C
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Application
Apoferritin from equine spleen has been used:
- to determine its partition coefficients in phase systems
- in NaCl for iron loading of cultured cells
- in in situ liquid scanning transmission electron microscope for imaging the interface of biology and nanotechnology
Biochem/physiol Actions
This protein shell of ferritin lacking iron is widely used for the calibration of gel filtration columns and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. It is very abundant in beta-cells of the pancreas where it acts as an anti-oxidant. When added to cultured endothelial cells apoferritin is taken up in a dose-responsive manner and appears to protect the cells from oxidant-mediated cytolysis.
Physical form
Solution in 0.135 M sodium chloride.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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