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A6531

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat

affinity isolated antibody, suspension

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MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

agarose conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

suspension

species reactivity

mouse

extent of labeling

≥5 mg per mL

technique(s)

Ouchterlony double diffusion: suitable
immunoprecipitation (IP): suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

capacity

0.4 mg/mL, resin binding capacity (mouse IgG)

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
M8645A1047M8642
species reactivity

mouse

species reactivity

-

species reactivity

mouse

species reactivity

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

Ouchterlony double diffusion: suitable, immunoprecipitation (IP): suitable

technique(s)

Ouchterlony double diffusion: suitable

technique(s)

direct ELISA: 1:7,000-1:21,000, western blot: suitable

technique(s)

Ouchterlony double diffusion: suitable

conjugate

agarose conjugate

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

form

suspension

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

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General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies bind to all mouse IgG subclasses.

Immunogen

IgG isolated from pooled normal mouse serum

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies have been used for immunoprecipiation assays. The product can also be used for Ouchterlony double diffusion applications.
Soluble antigens from meningococci extracts were mixed with ascitic fluid containing primary antibody and 50 μl of agarose-linked goat anti-mouse IgG for 5 hours at 4 °C.

Biochem/physiol Actions

IgG helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG2a of IgG has cytotropic properties and IgG2 molecules have the ability to activate complement.

Physical form

Suspension in 0.5 M NaCl containing preservative

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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