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A2909

Sigma-Aldrich

Rabbit IgG−Agarose

saline suspension

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MDL number:
NACRES:
NA.46

conjugate

agarose conjugate

Quality Level

form

saline suspension

extent of labeling

≥5 mg per mL

technique(s)

immunoprecipitation (IP): suitable

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

storage temp.

2-8°C

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This Item
A0919A8914A9294
Sigma-Aldrich

A2909

Rabbit IgG−Agarose

Sigma-Aldrich

A0919

Mouse IgG−Agarose

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

saline suspension

form

(Suspension in 0.5 M NaCl containing preservative.)

form

saline suspension

form

PBS suspension

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoelectrophoresis: suitable

technique(s)

immunoelectrophoresis: suitable

extent of labeling

≥5 mg per mL

extent of labeling

1 mg per mL

extent of labeling

~1 mg per mL

extent of labeling

~2.4 mg Ab per mL

matrix

cross-linked 4% beaded agarose

matrix

Cross-linked 4% beaded agarose

matrix

Cross-linked 4% beaded agarose

matrix

Cross-linked 4% beaded agarose

General description

IgGs are glycoprotein antibodies that modulate several immune responses. IgG-Agarose is an immunoadsorbent that can be used to purify antibodies, remove species specific cross-reacting antibodies, or remove contaminating antibodies from an antiserum preparation. Characteristically, cross-reacting antibodies may be removed from an antiserum preparation using an equal resin volume of IgG-Agarose. However, the resin to antiserum ratio will vary with individual applications. Immunoglobulin G (IgG) is part of the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively.

Application

Rabbit IgG antibody (20 μl/ml) crosslinked to agarose beads was used to isolate RAT tagged proteins from whole cell extracts of 293T cells.Rabbit IgG crosslinked to agarose beads were used for purification of tagged protein from mammalian whole cell extracts at 0.5 to 1.0mg total protein to 10 μl of beads.
Rabbit IgG-Agarose has been used for immunoprecipitation and affinity purification assays.

Physical form

suspension in 0.5 M NaCl containing preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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