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Key Documents

B3670

Sigma-Aldrich

Betacellulin human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

BTC

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About This Item

CAS Number:
163150-12-7
MDL number:
MFCD00676521
UNSPSC Code:
12352202
NACRES:
NA.32

product name

Betacellulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

biological source

human

Quality Level

100

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.1-0.6 ng/mL ED50

mol wt

protein 9.5 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P35070

storage temp.

−20°C

Gene Information

human ... BTC(685)

Related Categories

Application

This product has been used to study differentiation of pancreatic acinar cell line (AR42J) into insulin-secreting cells and has been linked to a role in the growth and differentiation of islet cells in human pancreas.

Biochem/physiol Actions

Betacellulin, and the other members of the EGF family of growth factors, binds with high affinity to both EGFR and to erbB-4. Overexpression of Betacellulin with erbB-3 and erbB-4 has been linked to human endometrial adenocarcinoma.

Caution

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

Preparation Note

This product was lyophilized from a 0.2 μm filtered solution of PBS containing 5 mg of bovine serum albumin. It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 1 μg/mL.

Analysis Note

The biological activity of this product is measured by an ability to stimulate H-thmidine incorporation in EGF-responsive mouse BALB/c 3T3 fibroblasts. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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D J Riese et al.
Oncogene, 12(2), 345-353 (1996-01-18)
Betacellulin is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the erbB gene family (erbB-1/epidermal growth factor receptor (EGFR), neu/erbB-2/HER2, erbB-3/HER3 and
R Srinivasan et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 5(10), 2877-2883 (1999-10-28)
The objective of this study was to determine the immunohistochemical expression of the c-erbB-3 and c-erbB-4 growth factor receptors and their principal ligands, the neuregulins and betacellulin, in normal endometrium and determine whether there was evidence of under- or overexpression
J Miyagawa et al.
Endocrine journal, 46(6), 755-764 (2000-03-21)
Betacellulin (BTC) purified from mouse beta cell tumor (betaTC-3) is a new member of the epidermal growth factor (EGF) family which can bind receptor tyrosine kinase, EGF receptor (erbB1) and erbB4. It has been demonstrated that proBTC mRNA was abundantly
Mi-Young Song et al.
Experimental & molecular medicine, 46, e98-e98 (2014-05-31)
Type 1 diabetes is an autoimmune disease caused by permanent destruction of insulin-producing pancreatic β cells and requires lifelong exogenous insulin therapy. Recently, islet transplantation has been developed, and although there have been significant advances, this approach is not widely

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