B3804
BCIP®/NBT-Blue Liquid Substrate System for Membranes
alkaline phosphatase substrate
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5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system
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General description
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.
Application
BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.
Caution
Stable at least one year at 2-8 °C. This substrate is light-sensitive.
Other Notes
Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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