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B9673

Sigma-Aldrich

1-Bromo-3-chloropropane

for isolation of RNA

All Photos(1)
Synonym(s):
1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide
Linear Formula:
Cl(CH2)3Br
CAS Number:
109-70-6
Molecular Weight:
157.44
Beilstein:
605278
EC Number:
203-697-1
MDL number:
MFCD00000998
PubChem Substance ID:
24892149
NACRES:
NA.52

grade

for molecular biology

Assay

≥98.5% (GC)

form

liquid

refractive index

n20/D 1.486 (lit.)

bp

144-145 °C (lit.)

density

1.592 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string

ClCCCBr

InChI

1S/C3H6BrCl/c4-2-1-3-5/h1-3H2

InChI key

MFESCIUQSIBMSM-UHFFFAOYSA-N

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312571.01692CDS003116
1-Bromo-3-chloropropane for isolation of RNA

Sigma-Aldrich

B9673

1-Bromo-3-chloropropane

1,2-Dibromo-3-chloropropane PESTANAL®, analytical standard

Supelco

31257

1,2-Dibromo-3-chloropropane

1-Chlorobutane for liquid chromatography LiChrosolv®

Supelco

1.01692

1-Chlorobutane

3-Bromo-4-chloropyridine AldrichCPR

Sigma-Aldrich

CDS003116

3-Bromo-4-chloropyridine

grade

for molecular biology

grade

analytical standard

grade

-

grade

-

assay

≥98.5% (GC)

assay

-

assay

≥99.8% (GC)

assay

-

form

liquid

form

-

form

liquid

form

liquid

refractive index

n20/D 1.486 (lit.)

refractive index

n/D 1.5542

refractive index

-

refractive index

-

bp

144-145 °C (lit.)

bp

60-65 °C/7.5 mmHg

bp

79 °C/1013 hPa

bp

-

density

1.592 g/mL at 25 °C (lit.)

density

2.081 g/mL at 25 °C

density

0.886 g/cm3 at 20 °C

density

-

General description

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Application

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:
  • feline teeth
  • mice brain and liver tissues
  • human ectocervical epithelial cells
  • placental samples
  • human mesenchymal stromal cells (hMSCs)

Features and Benefits

  • less toxic alternative to chloroform
  • compatible with TRI reagent for isolation of RNA with decreased DNA contamination

Other Notes

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

284.0 °F

Flash Point(C)

> 140 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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