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C0678

Sigma-Aldrich

Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse

clone VC1.1, ascites fluid, buffered aqueous solution

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Synonym(s):
Monoclonal Anti-HNK-1, Anti-CD57, Anti-N-CAM
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

VC1.1, monoclonal

form

buffered aqueous solution

contains

15 mM sodium azide

species reactivity

mouse, rat, feline, human

technique(s)

western blot: 1:4,000 using cerebral cortex extract

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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1 of 4

This Item
C9672S6324D8168
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

clone

VC1.1, monoclonal

clone

NCAM-0B11, monoclonal

clone

SNX6-76, monoclonal

clone

MANDYS8, monoclonal

Gene Information

human ... B3GAT1(27087)
mouse ... B3gat1(76898)
rat ... B3gat1(117108)

Gene Information

human ... NCAM1(4684), NCAM2(4685)
rat ... Ncam1(24586), Ncam2(288280)

Gene Information

human ... SNX6(58533)
mouse ... Snx6(72183)
rat ... Snx6(362738)

Gene Information

human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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General description

Monoclonal Anti-HNK-1/N-CAM (CD57) (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Human natural killer cell -1 (HNK-1), also referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP, is mapped to human chromosome 11q25. The encoded protein belongs to the glucuronyltransferase gene family. Neural cell-adhesion molecule (N-CAM) is expressed on all neuroepithelial cells from the time of neural induction in the early embryo. It is known that the sulfated carbohydrate HNK-1 epitope is carried by several surface molecules including a proportion of N-CAM isoforms.

Specificity

Recognizes the CD57/HNK-1 human myeloid cell associated surface glycoprotein. The epitope recognized is an N-linked carbohydrate which is present in a variety of glycoproteins and in some glycolipids. It is resistant to formalin fixation and paraffin embedding. VC1.1 antibody and the HNK-1 (Leu7) antibody inhibit the binding of each other. The antibody recognizes myelin associated glycoprotein in some species and a high molecular weight chondroitin sulphate proteoglycan.
5th Workshop: code no. NK 67

Immunogen

A homogenate of cat primary visual cortex (area 17)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used:
  • as primary antibody for western blot analysis
  • immunocytochemistry
  • immunocytochemical
  • immunohistological staining

Biochem/physiol Actions

HNK1 (human natural killer-1) is referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP. It is a member of the glucuronyltransferase gene family. Human natural killer-1 (HNK1) carbohydrate epitope is expressed on various cell adhesion molecules in the nervous system and plays a role in cell-cell and cell-substrate interactions. HNK-1 epitope stabilizes GluR2 on neuronal surface membranes and regulates cell surface stability of GluR2 by modulating the interaction with N-cadherin. It is also plays a role in cell migration and synaptic plasticity. HNK-1 epitope has been associated with the risk of metastasis. It is observed to be highly expressed in melanoma cell lines.

Target description

CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, about 60% of NK active cells and on a subset of T cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Ronald S Goldstein
Methods in molecular biology (Clifton, N.J.), 584, 367-385 (2009-11-13)
Traditional methods of studying the differentiation of human embryonic stem cells (hESCs) include generation of embryoid bodies, induced differentiation in vitro, and transplantation to immune-deficient mice. The chick embryo is a well-studied and accessible experimental system that has been used
Xiao-Tan Zhang et al.
Zygote (Cambridge, England), 26(6), 457-464 (2018-12-07)
SummaryFibroblast growth factor (FGF) signalling acts as one of modulators that control neural crest cell (NCC) migration, but how this is achieved is still unclear. In this study, we investigated the effects of FGF signalling on NCC migration by blocking
Loss of Shp2-mediated mitogen-activated protein kinase signaling in Muller glial cells results in retinal degeneration
Cai Z, et al.
Molecular and Cellular Biology, 31(14), 2973-2983 (2011)
Jean-Marie Gasc et al.
Biology open, 4(5), 666-671 (2015-04-04)
The enteric nervous system originates from neural crest cells that migrate in chains as they colonize the embryonic gut, eventually forming the myenteric and submucosal plexus. Failure of the neural crest cells to colonize the gut leads to aganglionosis in
Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells: induction, maintenance, and differentiation into functional schwann cells
Liu Q, et al.
Stem Cells Translational Medicine, 1(4), 266-278 (2012)

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