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Imprint® Ultra Chromatin Immunoprecipitation Kit, Without Controls

ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing

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General description

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.

application

  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

  • Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
  • Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
  • Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
  • Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Only

Product No.
Description

  • Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water

  • Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution

Kit Components Also Available Separately

Product No.
Description
SDS

  • S6576ChIP Next Gen Seq SepharoseSDS

  • I8896IGEPAL® CA-630, for molecular biologySDS

  • S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell cultureSDS

  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS

  • I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderSDS

  • I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderSDS

  • A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)SDS

  • A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)SDS

  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridizationSDS

  • M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionSDS

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS

  • R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)SDS

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signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

target_organs

Central nervous system

Storage Class

5.1A - Strongly oxidizing hazardous materials

wgk_germany

WGK 3


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Chromatin isolation by RNA purification (ChIRP).
Chu, C., et al.
Journal of Visualized Experiments (2012)
Comparison of sample preparation methods for ChIP-chip assays.
O'Geen, H., et al.
Biotechniques, 41, 557-557 (2006)
Genetic Framework for GATA Factor Function in Vascular Biology
Linnemann A.K., et al.
Proceedings of the National Academy of Sciences of the USA, 1, 13641-13646 (2011)
Philippe Collas
Molecular biotechnology, 45(1), 87-100 (2010-01-16)
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Chu, C., et al.
Molecular Cell (2011)

Protocols

Chromatin Immunoprecipitation quantitative real-time PCR (ChIP-qPCR) is commonly used in studies that focus on specific genes and potential regulatory regions across differing experimental conditions and data analysis. qPCR enables DNA analysis in real time by analyzing fluorescent signal intensities that are proportional to the amount of amplicon.

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Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

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