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F3013

Sigma-Aldrich

Ferredoxin from Spinacia oleracea (spinach)

lyophilized powder, Ferredoxin ≥15 wt. %

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CAS Number:
MDL number:
NACRES:
NA.61

biological source

spinach (Spinacia oleracea)

Quality Level

form

lyophilized powder

composition

Ferredoxin, ≥15 wt. %

application(s)

cell analysis

storage temp.

−20°C

Gene Information

spinach ... SOVF(137530)

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F5875F0628P9752
vibrant-m

P9752

Phosphoriboisomerase from spinach

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

form

partially purified powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C

Gene Information

spinach ... SOVF(137530)

Gene Information

spinach ... SOVF(137530)

Gene Information

-

Gene Information

-

composition

Ferredoxin, ≥15 wt. %

composition

-

composition

-

composition

-

General description

Ferredoxin I (Fd I) from Spinacia oleracea comprises 97 amino acid and is an iron sulfur cluster (2Fe-2S). It is synthesized in the chloroplast. The structure of Fd I has conserved active site.

Application

Ferredoxin from Spinacia oleracea (spinach) has been used:
  • as a substrate for spinach ferredoxin reductase,
  • for reconstitution with chlorophyll-A for scanning electron microscope (SEM) dispersive X-ray analyzer (EDX) integrated analysis,
  • as a reductant in adenylylsulfate (APS) reductase activity of P. aeruginosa

Biochem/physiol Actions

Ferredoxin is important in the electron transfer cascade required for this nitrogen assimilation. It has been used in studies of assimilation of inorganic nitrogen from nitrite or nitrate in the absence of ammonium.
Ferredoxins are iron-containing proteins that serve as electron-transfer catalysts in photosynthesis and nitrogen fixation.

Packaging

Package sizes are based on ferredoxin content.

Physical form

Contains Tris buffer

Preparation Note

Partially purified by the method of Tagawa and Arnon, Nature, 195, 537 (1962).

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar
Khalifa SAM, et al.
Parasite (Paris, France), 13(1), 51-58 (2006)
Structure of the Mutant E92K of [2Fe-2S] Ferredoxin I from Spinacia oleracea at 1.7 AA Resolution
Binda C, et al
Acta Crystallographica Section D, Biological Crystallography, 54(6), 1353-1358 (1998)
Keita Miyake et al.
The FEBS journal, 287(18), 4016-4031 (2020-01-30)
Bilin pigments play important roles for both light perception and harvesting in cyanobacteria by binding to cyanobacteriochromes (CBCRs) and phycobilisomes (PBS), respectively. Among various cyanobacteria, Acaryochloris marina MBIC 11017 (A. marina 11017) exceptionally uses chlorophyll d as the main photosynthetic pigment absorbing
Harnessing a P450 fatty acid decarboxylase from Macrococcus caseolyticus for microbial biosynthesis of odd chain terminal alkenes
Lee JW, et al.
Metabolic Engineering Communications, 7, e00076-e00076 (2018)
Rosa María Martínez-Espinosa et al.
FEMS microbiology letters, 277(1), 50-55 (2007-11-08)
Haloferax mediterranei is a halophilic archaeon that can grow using nitrate as the sole nitrogen source. A ferredoxin that serves as the physiological electron donor to the nitrate and nitrite reductases in this assimilatory process has been characterized. The ferredoxin

Protocols

Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).

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