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Key Documents

GE29-1487-23

Sigma-Aldrich

Superdex® Increase 75

3.2/300 GL, Cytiva 29-1487-23, column L × I.D. 30 cm × 3.2 mm, 9 μm avg. part. size

Synonym(s):

Superdex 75 increase

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About This Item

UNSPSC Code:
41115711
NACRES:
SB.52

material

glass column
titanium frit

product line

Superdex®

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

3000-70,000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 29-1487-23

parameter

<0.15 mL/min flow rate
4-50 μL sample volume

bed volume

~2.4 mL

column L × I.D.

30 cm × 3.2 mm

matrix

polymeric

avg. part. size

9 μm

working range

3-12

separation technique

size exclusion (SEC)

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General description

Our new generation columns called "Increase" offer higher resolution and shorter run times than their predecessors. Superdex 75 Increase is designed for small-scale preparative purification and analysis of recombinant tagged or other proteins with molecular weights from 3000 up to 70 000, such as recombinant tagged proteins

Features and Benefits

Comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity, Superdex 75 Increase columns offer:
  • Up to 50% higher resolution compared with predecessor Superdex 75, for improved purity and analysis results.
  • Runtime reduced down to one third compared with predecessor Superdex 75, with maintained resolution for results faster.
  • Versatile use in both preparative and analytical applications, especially with recombinant tagged proteins.
  • Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
  • Higher lot-to-lot consistency compared with predecessor Superdex 75.

Storage and Stability

Store at 4-30 °C (20% ethanol)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

104.9 °F

flash_point_c

40.5 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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