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HyStem®-C Cell Culture Scaffold Kit

For 7.5 mL of hydrogel scaffold solution


Quality Level

storage temp.


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General description

The HyStem® -C Cell Culture Scaffold Kit provides an excellent starting point for optimizing the matrix for stem cell culture. It is recommended for cultures, which require a minimal number of cell attachment sites or the addition of other extracellular matrix (ECM) proteins.


HyStem® -C cell culture scaffold kit has been used for neural stem cell culture.

Features and Benefits

Customizable, well characterized and consistent, Hystem-C hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
  • Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem-C offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
  • Synthetic - As a synthetic matrix, Hystem-C offers greater control of the cellular environment than ambiguous extracted ECM alternatives. All components are well characterized and consistently formulated. Hystem-C contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), and Water.
  • Biologically accurate - Rich in hyaluronic acid and denatured collagen, the Hystem-C hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.
Hystem-C enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

With Hystem-C, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem-C has been successfully used to expand human embryonic stem cells, human mesenchymal stem cells, neural progenitor cells and hepatic progenitor cells.

Legal Information

Hystem is a registered trademark of BIOTIME, INC


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Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

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Douglas O Sobel et al.
Islets, 12(4), 71-86 (2020-06-23)
Immortalized beta cells are an abundant source of insulin-producing cells. Although MIN-6 cells have similar characteristics as normal islets in vitro, the in vivo use of MIN-6 cells has not been fully described. This study characterizes in vivo mouse models
Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo
Basuodan R, et al.
Journal of Anatomy (2018)
Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo
Basuodan R, et al.
Journal of Anatomy (2018)
Reem Basuodan et al.
Journal of anatomy, 233(2), 155-166 (2018-05-11)
Human neural stem cells (hNSC) derived from induced pluripotent stem cells can be differentiated into neurons that could be used for transplantation to repair brain injury. In this study we dispersed such hNSC in a three-dimensional artificial extracellular matrix (aECM)


What is a Cell Delivery Vehicle?

Cell delivery vehicle are components that can be associated with cells and that allow transplantation in human or animals hosts. These can be natural or synthetic components that will form a gel, embedding the cells.

HyStem™ Hyaluronic Acid Based Hydrogels for 3D Cell Culture Applications

Hystem 3D hydrogels are based on hyaluronic acid, a major component of the extracellular matrix. 3D cell culture can be done using Hystem hydrogels.

Heparin Versus Heparin Sulfate Proteoglycans

Heparin is distinct from heparan sulfate proteoglycans (HS) which is primarily produced by mast cells. While, HS is produced by all cell types. Know more about the difference between heparin and heparin sulfate proteoglycan and select the right component for your study.

What is Hyaluronan?

Hyaluronan is a major component of the extracellular matrix. It’s the simplest glycosaminoglycan, and it plays several biological roles.

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