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TRUE3

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TrueGel3D Hydrogel Kits

FAST-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker

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dry ice

storage temp.

−70°C

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TRUE3

TrueGel3D Hydrogel Kits

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TRUE1

TrueGel3D Hydrogel Kits

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TRUE7

TrueGel3D Hydrogel Kits

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TRUE6

TrueGel3D Hydrogel Kits

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

General description

The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on CD cell-degradable crosslinker. Malemide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
TrueGel3D® provides a high degree of control in a 3D hydrogel environment, providing greater consistency. It enables to fine-tune the microenvironments around the tissues or cells under study.

Application

TRUE3-1KT is used for cell migration studies of amoeboid cells including neutrophils, dendritic cells and lymphocytes. It has been used to study the effect of mechanic stimulus over tumor development, tumor progression and cell differentiation.

Features and Benefits

  • Fast gelling (takes few seconds to minutes after addition of crosslinker)
  • Contains MMP-cleavable crosslinker
  • Allow cell recovery and post-culture analyses
  • Flexibility in modulating gel stiffness
  • Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)

Components

TRUE3-1KT contains lyophilized FAST-DEXTRAN (polymer) and CD cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.

Caution

Avoid prolonged exposure of CD cell-degradable crosslinker to air.

Legal Information

TRUEGEL3D is a registered trademark of Merck KGaA, Darmstadt, Germany

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Corrosion

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Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials


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How some labs put more bio into biomaterials.
Vivien Marx
Nature methods, 16(5), 365-368 (2019-05-02)

Articles

TrueGel3D™ synthetic hydrogel can be used to study co-culture of tumor and stromal cells.

The TrueGel3D™ hydrogel is formed by crosslinking RGD (arginylglycylaspartic acid) degradable polymer with CD cell-degradable crosslinker. Troubleshoot hydrogel formation and cell viability with this article.

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

Protocols

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimic the native tissue environment.

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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