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HYSHP020

Sigma-Aldrich

HyStem®-HP Cell Culture Scaffold Kit

For 7.5 mL of hydrogel scaffold solution

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NACRES:
NA.75

Quality Level

storage temp.

−20°C

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This Item
HYSHP010HYSC010HYS010
HyStem® Cell Culture Scaffold Kit Trial Kit, For 2.5 mL of hydrogel scaffold solution

HYS010

HyStem® Cell Culture Scaffold Kit

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

The HyStem®-HP Cell Culture Scaffold Kit provides an excellent starting point for optimizing the matrix for stem cell culture, where slowly released growth factors are crucial in re-creating a stem cell niche. To affect specific cell performance, growth factors or ECM proteins may be added to the HyStem-HP hydrogel. Unlike animal-derived ECM products, this kit contains three fully chemically defined components, which are nonimmunogenic:
  • HyStem®-HP – a thiol-modified hyaluronan (a major constituent of native ECM), carboxymethyl hyaluronic acid-thiopropanoyl hydrazide (CMHA-S, CMHA-DTPH, carboxymethyl hyaluronic acid-DTPH) with heparinthiopropanoyl hydrazide (HP-DTPH). HyStem-HP (CMHA-DTPH with HP-DTPH) contains 99.7 wt% CMHA-DTPH and 0.3 wt% HP-DTPH.
  • Gelin-S – a thiol-modified gelatin (denatured collagen), carboxymethyl gelatin-thiopropanoyl hydrazide (GTN-DTPH, carboxymethyl gelatin-DTPH)
  • Extralink® – a thiol-reactive crosslinker, polyethylene glycol diacrylate (MW = 3,400 g/mole, PEGDA)

Features and Benefits

Customizable, well characterized and consistent, Hystem-HP hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
  • Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem-HP offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
  • Synthetic - As a synthetic matrix, Hystem-HP offers greater control of the cellular environment than ambiguous extracted ECM alternatives. All components are well characterized and consistently formulated. Hystem-HP contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), Heparin and Water.
  • Biologically accurate - Rich in hyaluronic acid and denatured collagen, the Hystem-HP hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.
Hystem-HP enables ionic binding and slow release of growth factors through thiolated heparin; growth factors can be integrated via the matrix rather than cell culture medium. Hystem-HP also enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

With Hystem-HP, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem-HP is recommended for both in vivo and in vitro experimentation.

Legal Information

Extralink is a registered trademark of Glycosan BioSystems, Inc.
Gelin is a trademark of Biopharm Ltd.
Hystem is a registered trademark of BIOTIME, INC

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Danger

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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