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Key Documents

L4646

Sigma-Aldrich

Lipids Cholesterol Rich from adult bovine serum

liquid, sterile-filtered, BioReagent, suitable for cell culture, suitable for insect cell culture

Synonym(s):

Bovine Serum Lipids, Cholesterol Rich Lipids, Lipids from Bovine

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About This Item

UNSPSC Code:
12352211
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

200

product line

BioReagent

form

liquid

composition

Cholesterol, 9.0-11.0 g/L
Protein, 15.0-25.0 g/L

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

impurities

Adventitious viral agents, none detected (9 CFR; 113.53)
 IgG, none detected (RID)
 Microbial content, none detected (USP XXII)
 Mycoplasma, none detected (Barile method)
≤6.0 EU/mg cholesterol endotoxin

storage temp.

2-8°C

Related Categories

General description

Cholesterol is a lipid molecule, found integrated to the cell membrane.

Application

Lipids Cholesterol Rich from adult bovine serum has been used as one of the lipid component to generate erythroblasts ex vivo. It has also been used as a standard to determine muscle cholesterol level, spectrophotometrically.

Biochem/physiol Actions

Cholesterol significantly affects the property of phospholipid membrane such as fluidity and membrane protein function. It exhibits a condensing effect towards phospholipids. Cholesterol-phospholipid interaction influences physiological properties of a lipid bilayer. Cholesterol serves as a precursor of bile acids, steroid hormones and vitamin D. Uncontrolled level of plasma cholesterol might lead to the risk of cardiovascular disorder.

Other Notes

Aqueous lipoprotein solution

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Humanized culture medium for clinical expansion of human erythroblasts
Migliaccio G, et al.
Cell Transplantation, 19(4), 453-469 (2010)
Dietary cholesterol: from physiology to cardiovascular risk
Lecerf JM and De Lorgeril M
The British Journal of Nutrition, 106(1), 6-14 (2011)
A molecular view of the cholesterol condensing effect in DOPC lipid bilayers
Alwarawrah M, et al.
The Journal of Physical Chemistry B, 114(22), 7516 -77523 (2010)
Influence of dietary canola oil and palm oil blend and refrigerated storage on fatty acids, myofibrillar proteins, chemical composition, antioxidant profile and quality attributes of semimembranosus muscle in goats
Adeyemi KD, et al.
Journal of Animal Science and Biotechnology, 6(1), 51-51 (2015)
Effect of cholesterol on the structure of a phospholipid bilayer
de Meyer F and Smit B
Proceedings of the National Academy of Sciences of the USA, 106(10), 3654-3658 (2009)
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