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L4646

Sigma-Aldrich

Lipids Cholesterol Rich from adult bovine serum

liquid, sterile-filtered, BioReagent, suitable for cell culture, suitable for insect cell culture

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About This Item

UNSPSC Code:
12352211
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

product line

BioReagent

form

liquid

composition

Cholesterol, 9.0-11.0 g/L
Protein, 15.0-25.0 g/L

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

impurities

Adventitious viral agents, none detected (9 CFR; 113.53)
IgG, none detected (RID)
Microbial content, none detected (USP XXII)
Mycoplasma, none detected (Barile method)
≤6.0 EU/mg cholesterol endotoxin

storage temp.

2-8°C

General description

Cholesterol is a lipid molecule, found integrated to the cell membrane.

Application

Lipids Cholesterol Rich from adult bovine serum has been used as one of the lipid component to generate erythroblasts ex vivo. It has also been used as a standard to determine muscle cholesterol level, spectrophotometrically.

Biochem/physiol Actions

Cholesterol significantly affects the property of phospholipid membrane such as fluidity and membrane protein function. It exhibits a condensing effect towards phospholipids. Cholesterol-phospholipid interaction influences physiological properties of a lipid bilayer. Cholesterol serves as a precursor of bile acids, steroid hormones and vitamin D. Uncontrolled level of plasma cholesterol might lead to the risk of cardiovascular disorder.

Other Notes

Aqueous lipoprotein solution

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effect of cholesterol on the structure of a phospholipid bilayer
de Meyer F and Smit B
Proceedings of the National Academy of Sciences of the USA, 106(10), 3654-3658 (2009)
Fatty acid profile, cholesterol and oxidative status in broiler chicken breast muscle fed different dietary oil sources and calcium levels
Abdulla NR, et al.
South African Journal of Animal Science, 45(2), 153-163 (2015)
The condensing effect of cholesterol in lipid bilayers
Hung WC, et al.
Biophysical Journal, 92(11), 3960-3967 (2007)
Paoletti R. and Kritchevsky D.
Advances in Lipid Research, 8, 1-1 (2014)
Humanized culture medium for clinical expansion of human erythroblasts
Migliaccio G, et al.
Cell Transplantation, 19(4), 453-469 (2010)

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