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M2933

Sigma-Aldrich

MES hydrate

BioPerformance Certified, suitable for cell culture, ≥99.5%

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Synonym(s):
2-Morpholineethanesulfonic acid hydrate, 2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid
Empirical Formula (Hill Notation):
C6H13NO4S · xH2O
CAS Number:
Molecular Weight:
195.24 (anhydrous basis)
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

Quality Level

Assay

≥99.5%

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and Total Aerobic Microbial Count, tested

useful pH range

5.5-6.7

pKa 

6.1

application(s)

diagnostic assay manufacturing

foreign activity

DNase, RNase, protease, none detected

SMILES string

O.OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2

InChI key

MIIIXQJBDGSIKL-UHFFFAOYSA-N

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1 of 4

This Item
M8250PHG0003RDD030
MES hydrate BioPerformance Certified, suitable for cell culture, ≥99.5%

Sigma-Aldrich

M2933

MES hydrate

Premium Grade
MES hydrate ≥99.5% (titration)

Sigma-Aldrich

M8250

MES hydrate

-
MES hydrate

SAFC

PHG0003

MES hydrate

-
MES hydrate free-flowing, Redi-Dri™, ≥99.0%

Sigma-Aldrich

RDD030

MES hydrate

-
assay

≥99.5%

assay

≥99.5% (titration)

assay

-

assay

≥99.0%

form

crystalline powder

form

crystalline powder

form

powder

form

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

impurities

endotoxin and Total Aerobic Microbial Count, tested

impurities

-

impurities

Endotoxin, microbial, and trace metals; tested

impurities

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

General description

MES hydrate buffer (2-(N-morpholino)ethanesulfonic acid monohydrate) is a versatile zwitterionic biological buffer widely utilized in molecular biology and cell culture applications. With a pKa of 6.1, it′s the ideal choice for buffering solutions at physiological pH, ensuring precise and reliable results. This buffer′s high water solubility and minimal metal ion binding make it a top choice for various applications, including molecular biology tasks such as DNA and RNA extraction, PCR, and gel electrophoresis. It′s also a key player in cell culture, offering a less toxic alternative to Tris and phosphate buffers.

Beyond these applications, MES hydrate buffer is widely used in regulating pH in plant culture media, reagent solutions, and physiological experiments. It′s the preferred choice for studying the effects of pH on enzymatic reactions and investigating the interactions of proteins and other biomolecules with metal ions. As a Good′s buffer, MES hydrate meets stringent criteria of having a midrange pKa, maximum water solubility, minimal solubility in other solvents, minimal salt effects, stability across different temperatures, chemical and enzymatic stability, minimal absorption in the visible and UV spectral range, and ease of synthesis. Furthermore, it does not form complexes with most metal ions, ensuring reliable outcomes in applications involving metal ions.

Application

MES Hydrate has been used:
  • To suspend cells before autophagic induction studies
  • In the preparation of Murashige and Skoog growth medium for the growth of Arabidopsis thaliana seedlings
  • In the conjugation of hybridization probes to beads before PCR amplification
  • To treat fibroblast-derived matrix before conjugation with heparin for use as a vascular endothelial growth factor delivery platform
  • as a wash buffer in a study about molecular biology
  • as a component of culture media

Features and Benefits

  • Suitable for Molecular Biology and Cell Culture
  • Can be used as a Buffer component, for Electrophoresis and Protein separation
  • Tested for Endotoxins and Total Aerobic Microbial Count
  • Free from DNase, NICKase, RNase, and Protease
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
  • Highly soluble in water
  • Minimal metal ion binding
  • Less toxic to cells than other buffers such as Tris and phosphate
  • Stable in a wide pH range
  • Low UV absorptivity
  • Minimal reactivity

Preparation Note

A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.

Storage and Stability

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

Easily compare specifications for MES products with the MES specification table.
Solubility: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. Similar product MES hydrate M8250 is tested at 20g/80mL water, or 1.3M. The pH of a solution should be between 2.5 and 5, depending on the concentration. A saturated solution at 0°C is approximately 0.65M.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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T1503
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25G
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705578-5MG-PW

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MMYOMAG-74K-13

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  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

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Fibroblast-derived matrix (FDM) as a novel vascular endothelial growth factor delivery platform.
Du, Ping, et al.
J. Controlled Release, 194, 122-129 (2014)
Autophagic or necrotic cell death in the absence of caspase and bcl-2 family members.
Lam, David, et al.
Biochemical and biophysical research communications, 363 (3), 536-541 (2007)
Dawson, R.M.C. et al.
Data for Biochemical Research, 410-410 (1987)
Multiplex assay for subtyping avian influenza A viruses by cDNA hybridization and adapter-mediated amplification.
Applied Microbiology and Biotechnology, 100 (20), 8809-8818 (2016)
Protocol: An improved high-throughput method for generating tissue samples in 96-well format for plant genotyping (Ice-Cap 2.0).
Plant methods, 3 (1), 8-8 (2007)

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