M5287
MES hydrate
≥99.5% (titration), pH 2.5-4.0 (0.5 M in H2O), BioXtra
Synonym(s):
2-Morpholineethanesulfonic acid hydrate, 2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid
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About This Item
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Quality Level
product line
BioXtra
assay
≥99.5% (titration)
form
crystalline powder
storage condition
dry at room temperature
impurities
Insoluble matter, passes filter test
ign. residue (900°C)
≤0.05%
color
white
pH
2.5-4.0 (0.5 M in H2O)
useful pH range
5.5-6.7
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General description
MES hydrate buffer also exhibits high water solubility and minimal metal ion binding, making it an ideal choice for various applications:
- Cell Biology: MES hydrate buffer is often the preferred choice for buffering cell culture media due to its lower toxicity to cells compared to other buffers like Tris and phosphate.
- Protein Purification and Extraction: Its stability across a wide pH range and minimal metal ion binding make MES hydrate buffer a valuable asset in protein purification and extraction protocols.
- Gel Electrophoresis: MES hydrate buffer is also commonly used as a running buffer in denaturing gel electrophoresis, ensuring reliable results in protein separation and analysis.
application
- As a component of extraction buffer in the extraction of the enzyme BACE1[1]
- As a component of the reaction mixture for the measurement of 5,10-methenyltetrahydrofolate synthetase (MTHFS) enzyme activity in fibroblasts[2]
- As a component of MES coupling buffer during the coupling of mAb or GST-fusion proteins to carboxylate-modified polystyrene latex (CML) beads[3]
- to adjust the pH of the solution for the fluorescent labeling[4]
- as a component of electrophoresis buffer[5]
Features and Benefits
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
- Highly soluble in water
- Minimal metal ion binding
- Less toxic to cells than other buffers such as Tris and phosphate
- Stable in a wide pH range
- Low UV absorptivity
- Minimal reactivity
Preparation Note
Storage and Stability
Other Notes
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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