M5287

MES hydrate

≥99.5% (titration), pH 2.5-4.0 (0.5 M in H₂O), BioXtra

• Synonym(s): 2-Morpholineethanesulfonic acid hydrate, 2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid
• Empirical Formula (Hill Notation): C₆H₁₃NO₄S · xH₂O
• CAS Number: 1266615-59-1
• Molecular Weight: 195.24 (anhydrous basis)
• MDL number: MFCD00149409
• UNSPSC Code: 12352106
• eCl@ss: 32129211
• PubChem Substance ID: 329818007
• NACRES: NA.25

Properties

• product line: BioXtra
• assay: ≥99.5% (titration)
• form: crystalline powder
• storage condition: dry at room temperature
• impurities: Insoluble matter, passes filter test
• ign. residue (900°C): ≤0.05%
• color: white
• pH: 2.5-4.0 (0.5 M in H₂O)
• useful pH range: 5.5-6.7
• pKa: 6.1
• solubility: H₂O: 0.5 M, clear, colorless; water: 335.3 g/L at 20 °C
• anion traces: chloride (Cl^-): ≤0.005%
• cation traces: Al: ≤0.0005%; As: ≤0.0001%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.002%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.005%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• absorption: ≤0.020 at 280 in H₂O at 0.5 M; ≤0.025 at 260 in H₂O at 0.5 M
• application(s): cell analysis; life science and biopharma; sample preparation
• storage temp.: room temp
• SMILES string: O.OS(=O)(=O)CCN1CCOCC1
• InChI: 1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
• InChI key: MIIIXQJBDGSIKL-UHFFFAOYSA-N

Description

General description

MES hydrate buffer (2-(N-morpholino)ethanesulfonic acid monohydrate) is a zwitterionic biological buffer widely employed in biological, environmental, and biochemical research. With a pKa of 6.1, it is the go-to choice for buffering solutions at physiological pH. MES was developed as one of Good′s buffers in the 1960s, meeting specific criteria: a midrange pKa, maximum water solubility, minimal solubility in other solvents, minimal salt effects, stability in different temperatures, chemical and enzymatic stability, minimal absorption in the visible and UV spectral range, and ease of synthesis. MES Monohydrate′s attributes, such as low UV absorptivity, minimal reactivity, pH stability, and water solubility, position it as a Good′s buffer.

MES hydrate buffer also exhibits high water solubility and minimal metal ion binding, making it an ideal choice for various applications:

• Cell Biology: MES hydrate buffer is often the preferred choice for buffering cell culture media due to its lower toxicity to cells compared to other buffers like Tris and phosphate.
• Protein Purification and Extraction: Its stability across a wide pH range and minimal metal ion binding make MES hydrate buffer a valuable asset in protein purification and extraction protocols.
• Gel Electrophoresis: MES hydrate buffer is also commonly used as a running buffer in denaturing gel electrophoresis, ensuring reliable results in protein separation and analysis.

Application

MES Hydrate has been used:

• As a component of extraction buffer in the extraction of the enzyme BACE1
• As a component of the reaction mixture for the measurement of 5,10-methenyltetrahydrofolate synthetase (MTHFS) enzyme activity in fibroblasts
• As a component of MES coupling buffer during the coupling of mAb or GST-fusion proteins to carboxylate-modified polystyrene latex (CML) beads
• to adjust the pH of the solution for the fluorescent labeling
• as a component of electrophoresis buffer

Features and Benefits

• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
• Highly soluble in water
• Minimal metal ion binding
• Less toxic to cells than other buffers such as Tris and phosphate
• Stable in a wide pH range
• Low UV absorptivity
• Minimal reactivity

Preparation Note

A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.

Storage and Stability

Solutions are stable at 2-8 C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

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Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves