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M3058

Sigma-Aldrich

MES sodium salt

BioPerformance Certified, suitable for cell culture

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Synonym(s):
4-Morpholineethanesulfonic acid, sodium salt, Sodium 2-Morpholinoethanesulfonate, 2-(N-Morpholino)ethanesulfonic acid sodium salt, 4-Morpholineethanesulfonic acid sodium salt
Empirical Formula (Hill Notation):
C6H12NNaO4S
CAS Number:
Molecular Weight:
217.22
Beilstein:
3765682
EC Number:
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

useful pH range

5.5-6.7

pKa 

6.1

application(s)

diagnostic assay manufacturing

foreign activity

DNase, NICKase, RNase, protease, none detected

SMILES string

[Na+].[O-]S(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1

InChI key

IRHWMYKYLWNHTL-UHFFFAOYSA-M

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This Item
M5057M3885RES0114M-A7
MES sodium salt BioPerformance Certified, suitable for cell culture

Sigma-Aldrich

M3058

MES sodium salt

Premium Grade
MES sodium salt BioReagent, ≥99% (titration), crystalline

Sigma-Aldrich

M5057

MES sodium salt

Essential+ Grade
MES sodium salt ≥99% (titration)

Sigma-Aldrich

M3885

MES sodium salt

-
MES sodium salt

SAFC

RES0114M-A7

MES sodium salt

-
form

crystalline powder

form

crystalline

form

crystalline powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

impurities

-

impurities

≤8% water content (Karl Fischer)

impurities

Endotoxin and microbial, tested

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

foreign activity

DNase, NICKase, RNase, protease, none detected

foreign activity

-

foreign activity

-

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

General description

4-Morpholineethanesulfonic acid, sodium salt (MES) is a popular zwitterionic buffer in scientific research due to its excellent properties. It can buffer solutions effectively within a pH range of 5.5-6.5 with a pKa value of 6.1. This product is BioPerformance certified and it finds extensive application as a buffering agent in biochemistry and molecular biology experiments, contributing to the stabilization of pH in different biological reactions including western blotting, cell culture and electrophoresis.
MES is one of the so-called "Good" buffers developed for biological applications. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Application

MES sodium salt has been employed as a coupling buffer for amine-reactive sensors at either pH 4.5 or pH 5.0 to facilitate the coupling of monoclonal antibodies (MAbs) onto the sensors, used in hybridization studies with fluorescently-labeled complementary strands and microarray slide washing during microarray analysis, and played a role in preparing a new set of RNA phosphoramidites compatible with in situ photolithography for growing RNA oligonucleotides on microarrays.
MES sodium salt has been used as a coupling buffer during the coupling of MAbs onto amine-reactive sensors. It has also been used as a component of germination medium and shoot induction medium during in vitro plant regeneration.

Features and Benefits

  • Suitable for Molecular Biology and Cell Culture
  • Tested for Endotoxins and Total Aerobic Microbial Count
  • Free from DNase, NICKase, RNase, and Protease
  • Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)

Preparation Note

MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions are stable at 2-8°C for months. Sterilization should be done by filteration through 0.2μm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

Easily compare specifications for MES sodium salt products with the MES sodium salt specification table.

comparable product

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

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High-throughput epitope binning assays on label-free array-based biosensors can yield exquisite epitope discrimination that facilitates the selection of monoclonal antibodies with functional activity.
Abdiche, YN et al.
PLoS ONE, 9 (3), e92451-e92451 (2014)
In vitro plant regeneration from commercial cultivars of soybean.
Raza, G
Biomedical Research, 2017 (2017)
Yasmina Noubia Abdiche et al.
PloS one, 9(3), e92451-e92451 (2014-03-22)
Here, we demonstrate how array-based label-free biosensors can be applied to the multiplexed interaction analysis of large panels of analyte/ligand pairs, such as the epitope binning of monoclonal antibodies (mAbs). In this application, the larger the number of mAbs that
High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
Lietard J, et al.
Journal of Visualized Experiments, 2019, 150-150 (2019)
Dawson, RMC et al.
Data for Biochemical Research, 410-410 (1987)

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