MilliporeSigma
All Photos(4)

Documents

M5057

Sigma-Aldrich

MES sodium salt

BioReagent, ≥99% (titration), crystalline

Sign Into View Organizational & Contract Pricing

Synonym(s):
2-(N-Morpholino)ethanesulfonic acid sodium salt, 4-Morpholineethanesulfonic acid sodium salt
Empirical Formula (Hill Notation):
C6H12NNaO4S
CAS Number:
Molecular Weight:
217.22
Beilstein:
3765682
EC Number:
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

product line

BioReagent

Quality Level

Assay

≥99% (titration)

form

crystalline

pH

8.9-10.1 (1% in water)

useful pH range

5.5-6.7

pKa 

6.1

solubility

water: 0.5 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

storage temp.

room temp

SMILES string

[Na+].[O-]S(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1

InChI key

IRHWMYKYLWNHTL-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
M3058M3885RES0114M-A7
MES sodium salt BioReagent, ≥99% (titration), crystalline

Sigma-Aldrich

M5057

MES sodium salt

Essential+ Grade
MES sodium salt BioPerformance Certified, suitable for cell culture

Sigma-Aldrich

M3058

MES sodium salt

Premium Grade
MES sodium salt ≥99% (titration)

Sigma-Aldrich

M3885

MES sodium salt

-
MES sodium salt

SAFC

RES0114M-A7

MES sodium salt

-
assay

≥99% (titration)

assay

-

assay

≥99% (titration)

assay

-

form

crystalline

form

crystalline powder

form

crystalline powder

form

powder

pH

8.9-10.1 (1% in water)

pH

-

pH

-

pH

-

solubility

water: 0.5 g/mL, clear, colorless

solubility

-

solubility

water: 0.5 g/mL, clear, colorless

solubility

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

General description

MES is one of the so-called "Good" buffers developed for biological applications. This product is tested for trace ions. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±-1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Application

MES sodium salt has been used:
  • To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNA
  • To equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization

Preparation Note

MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions should be stable at 2-8°C for months. Sterilization should be by filteration through 0.2μm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

Easily compare specifications for MES sodium salt products with the MES sodium salt specification table.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

MES hemisodium salt dry powder

Sigma-Aldrich

M0164

MES hemisodium salt

MES solution BioUltra, for molecular biology, 0.5 M in H2O

Sigma-Aldrich

76039

MES solution

MES hydrate BioPerformance Certified, suitable for cell culture, ≥99.5%

Sigma-Aldrich

M2933

MES hydrate

MES monohydrate

SAFC

RES0113M-A7

MES monohydrate

MES solution suitable for, BioPerformance Certified, 1 M, suitable for cell culture

Sigma-Aldrich

M1317

MES solution

BIS-TRIS propane BioPerformance Certified, suitable for cell culture, ≥99.0%

Sigma-Aldrich

B4679

BIS-TRIS propane

MES hemisodium salt ≥98% (titration)

Sigma-Aldrich

M8902

MES hemisodium salt

Dawson, RMC et al.
Data for Biochemical Research, 410-410 (1987)
Good NE et al.
Biochemistry, 5, 467-477 (1966)
Lucia Falbo et al.
Nature communications, 11(1), 1345-1345 (2020-03-14)
In several metazoans, the number of active replication origins in embryonic nuclei is higher than in somatic ones, ensuring rapid genome duplication during synchronous embryonic cell divisions. High replication origin density can be restored by somatic nuclear reprogramming. However, mechanisms
Takamitsu Miyafusa et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 64(Pt 6), 512-515 (2008-06-10)
Capsular polysaccharides (CPs) are important virulence factors of Staphylococcus aureus. The biosynthesis of type 5 and type 8 CPs (CP5 and CP8), which are produced by most clinical isolates of S. aureus, is catalyzed by 16 CP-assembling proteins. One of
Tsuyoshi Shirai et al.
Journal of molecular biology, 321(5), 879-889 (2002-09-11)
The crystal structure of congerin II, a galectin family lectin from conger eel, was determined at 1.45A resolution. The previously determined structure of its isoform, congerin I, had revealed a fold evolution via strand swap; however, the structure of congerin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service