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MAK092

Sigma-Aldrich

Peroxidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

storage temp.

−20°C

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MAK166MAK311CGP1
Peroxide Assay Kit sufficient for 250 colorimetric tests

Sigma-Aldrich

MAK311

Peroxide Assay Kit

detection method

colorimetric, fluorometric

detection method

fluorometric

detection method

colorimetric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

General description

Peroxidase (EC 1.11.1.7) is an enzyme found broadly in biological systems that utilizes hydrogen peroxide in the oxidation of various substrates. The Peroxidase Activity Assay Kit provides a simple and direct procedure for measuring peroxidase activity in a variety of biological samples.

Application

Peroxidase Activity Assay Kit has been used to quantify the activity of peroxidase.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the determination of peroxidase activity in cell culture supernatant, serum, plasma, urine, and other biological fluids.

Principle

Peroxidase catalyzes the reaction between H2O2 and the probe, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the peroxidase activity present. One unit of peroxidase is defined as the amount of enzyme that reduces1.0 μmole of H2O2 per minute at 37°C.

replaced by

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup


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Protocols

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

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