MTOX1005
MDR1/MRP2 Double Knockout Caco-2 Cells
Human male colorectal tissue, adenocarcinoma
Synonym(s):
C2BBe1 Cells MDR1/MRP2 (-/-/-/-,-/-/-/-)
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
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product name
MDR1/MRP2 Double Knockout Caco-2 Cells, one vial
biological source
human male colorectal tissue (Source disease: adenocarcinoma)
form
liquid
technique(s)
drug transporter assay: suitable
permeability assay: suitable
application(s)
ADME/TOX
storage temp.
−196°C
Gene Information
human ... ABCB1(5243) , ABCC2(1244)
General description
The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 and MRP2 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional double knockout of the ABCB1 and ABCC2 (MDR1/MRP2) efflux transporters.
Application
The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Features and Benefits
The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:
- A functional double knockout of the MDR1 gene and MRP2 gene eliminates the reliance on chemical inhibitors to determine if a compound is an BCRP substrate
- The vial format enables the MDR1 and MRP2 knockout cells to be included in standard drug transporter protocols
- Human assay with no interference from animal inhibitors
- Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality
Legal Information
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Kit Components Also Available Separately
Product No.
Description
SDS
- MTOX1005MDR1/MRP2 Double Knockout Caco-2 Cells, one vial 1 vialSDS
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Angelo E Andres et al.
Methods in molecular biology (Clifton, N.J.), 2430, 449-466 (2022-04-28)
Taxoids such as paclitaxel (Taxol) are an important class of anticancer drugs that bind β-tubulin and stabilize cellular microtubules. To provide new chemical tools for studies of microtubules, we synthesized derivatives of paclitaxel modified at the 7-position with the small
P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
Mark I Kaldas et al.
The Journal of pharmacy and pharmacology, 55(3), 307-312 (2003-05-02)
Resveratrol is a dietary constituent suggested to have protective effects against cancer as well as cardiovascular disease. The purpose of the study was to learn whether this agent could be absorbed in man and enter the systemic circulation. This was
M J Briske-Anderson et al.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 214(3), 248-257 (1997-03-01)
The Caco-2 cell line is used by many investigators as a model of the intestinal epithelium to study nutrient uptake and transport. Our goal was to create an awareness of inherent variabilities in the Caco-2 cell line which may influence
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
Articles
We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.
Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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