MWGF70

Gel Filtration Markers Kit for Protein Molecular Weights 6,500-66,000 Da

• Synonym(s): Gel Filtration Protein Ladder
• NACRES: NA.25
• UNSPSC Code: 12352200

Properties

• Quality Level: 200
• form: solid
• technique(s): protein quantification: suitable
• storage temp.: −20°C

Description

General description

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins. Fractionation is based on the diffusion of molecules into the pores of the resin. Larger proteins do not enter the pores of the resin as readily but pass through the fluid volume of the column faster than smaller proteins. These protein molecules elute from the column in order of decreasing molecular mass.

Application

Gel filtration markers are used in protein chromatography and gel filtration chromatography. Gel filtration markers have been used to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system. Gel filtration markers have also been used to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner.

Gel Filtration Markers Kit for Protein Molecular Weights 6,500-66,000 Da has been used as a standard in size exclusion chromatography.

Kit Components Also Available Separately

• A8531

  Albumin, Bovine serum, 66,000 MW 1 mL/vial

  SDS
• A3886

  Aprotinin, Bovine lung, 6,500 MW 1 mL/vial

  SDS
• D4772

  Blue Dextran 2,000,000 MW 1 mL/vial

  SDS
• C7025

  Carbonic Anhydrase, Bovine erythrocytes, 29,000 MW 1 mL/vial

  SDS
• C7150

  Cytochrome c, Horse heart, 12,400 MW 1 mL/vial

  SDS

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3