MilliporeSigma
All Photos(1)

Documents

RAB0522

Sigma-Aldrich

Bovine TNFα / Tumor Necrosis Factor alpha ELISA Kit

for serum, plasma and cell culture supernatants

Sign Into View Organizational & Contract Pricing

NACRES:
NA.32

species reactivity

bovine

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 0.1 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

bovine ... TNF(280943)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
RAB0526RAB0478RAB0855
Gene Information

bovine ... TNF(280943)

Gene Information

canine ... TNF(403922)

Gene Information

Porcine ... TNF(397086)

Gene Information

mouse ... Tnfrsf19(29820)

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 0.1 ng/mL

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 2 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 0.014-10 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.24 ng/mL
standard curve range: 0.246-60 ng/mL

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

This ELISA antibody pair detects bovine Tumor Necrosis Factor.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

F Zandkarimi et al.
Journal of dairy science, 101(6), 5531-5548 (2018-03-27)
Clinical mastitis (CM), the most prevalent and costly disease in dairy cows, is diagnosed most commonly shortly after calving. Current indicators do not satisfactorily predict CM. This study aimed to develop a robust and comprehensive mass spectrometry-based metabolomic and lipidomic
Syed A H Zaidi et al.
Investigative ophthalmology & visual science, 61(11), 17-17 (2020-09-12)
We determined whether δ-opioid receptor agonist (SNC-121) regulates acetylation homeostasis via controlling histone deacetylases (HDACs) activity and expression in optic nerve head (ONH) astrocytes. ONH astrocytes were treated with SNC-121 (1 µM) for 24 hours. The HDAC activity was measured
Jinsu Hong et al.
Journal of animal science, 98(11) (2020-10-25)
An experiment was conducted to determine the effects of including canola meal (CM) in nursery pig diets on growth performance, immune response, fecal microbial composition, and gut integrity. A total of 200 nursery pigs (initial body weight = 7.00 kg)
Horng-Tay Tzeng et al.
PloS one, 9(7), e103008-e103008 (2014-07-23)
Persistent hepatitis B viral (HBV) infection results in chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC). An efficient control of virus infections requires the coordinated actions of both innate and adaptive immune responses. In order to define the role of
Cormac T Taylor et al.
Biochemical and biophysical research communications, 447(4), 660-665 (2014-04-24)
Intermittent hypoxia (IH)-induced activation of pro-inflammatory pathways is a major contributing factor to the cardiovascular pathophysiology associated with obstructive sleep apnea (OSA). Obesity is commonly associated with OSA although it remains unknown whether adipose tissue is a major source of

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service