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S5393

Sigma-Aldrich

Serum minus IgA/IgM/IgG human

lyophilized powder

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NACRES:
NA.46

biological source

human

Quality Level

300

conjugate

unconjugated

form

lyophilized powder

contains

15 mM sodium azide

packaging

vial of 0.5 mL

storage temp.

2-8°C

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S5393

Serum minus IgA/IgM/IgG human

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I5381

IgG from mouse serum

IgG from rabbit serum reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

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IgG from bovine serum reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

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conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

form

lyophilized powder

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

packaging

vial of 0.5 mL

packaging

-

packaging

-

packaging

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

contains

15 mM sodium azide

contains

-

contains

-

contains

-

General description

Serum is the liquid component of blood that includes proteins, immunoglobulin and electrolytes. The product can be used for selective removal of contaminating antibodies when immobilized on a solid matrix. This product can also be used in anti-protective antigen (anti-PA) IgG measurements using PA coupled microspheres. Human serum deficient of IgG, IgA, and IgM can react specifically with anti-human whole serum but does not show reactivity with anti-human IgG, IgA, or IgM.

Application

Serum minus IgA/IgM/IgG human has been used as a control serum in immunoelectrophoresis, immunodiffusion,enzyme linked immunosorbent assay. It has also been used as
  • control sera to assess neuraminidase inhibition
  • microneutralization assay
  • single radial hemolysis (SRH) repeatability assay

Quantity

Each vial contains at least 25 mg protein

Reconstitution

Reconstitute with 0.5 mL deionized water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Karina Jawinski et al.
Frontiers in immunology, 10, 2661-2661 (2019-12-05)
Current influenza vaccines manufactured using eggs have considerable limitations, both in terms of scale up production and the potential impact passaging through eggs can have on the antigenicity of the vaccine virus strains. Alternative methods of manufacture are required, particularly
Fabrizio Biuso et al.
Influenza and other respiratory viruses, 13(5), 504-516 (2019-08-15)
Formulation of neuraminidase (NA) within influenza vaccines is gaining importance in light of recent human studies. The enzyme-linked lectin assay (ELLA) is considered a reliable assay to evaluate human anti-NA antibodies. To overcome interference by hemagglutinin (HA)-specific antibodies and detect
Thorunn A Olafsdottir et al.
Frontiers in immunology, 8, 1872-1872 (2018-01-24)
Influenza vaccination remains the best strategy for the prevention of influenza virus-related disease and reduction of disease severity and mortality. However, there is large individual variation in influenza vaccine responses. In this study, we investigated the effects of gender, age
Raymond E Biagini et al.
Clinical and diagnostic laboratory immunology, 10(5), 744-750 (2003-09-11)
We describe a fluorescent covalent microsphere immunoassay (FCMIA) method for the simultaneous (multiplexed) measurement of immunoglobulin G (IgG) antibodies to 23 pneumococcal capsular polysaccharide (PnPS) serotypes present in the pneumococcal polysaccharide vaccine (PPV23) licensed by the Food and Drug Administration
Validation of Single Radial Haemolysis assay: A reliable method to measure antibodies against influenza viruses
Trombetta CM, et al.
Journal of Immunological Methods, 422, 95-101 (2015)
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