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SAB4200689

Sigma-Aldrich

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

clone 5C5, hybridoma cell culture supernatant

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Synonym(s):
Actin alpha sarcomeric/skeletal, a-SCA, alpha-SCA, alpha-sarcomeric actin
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5C5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus

packaging

antibody small pack of 25 μL

technique(s)

ELISA: suitable
immunoblotting: 1:500-1:1000 using rat heart tissue extracts
immunofluorescence: 1:500-1000 using human HeLa cells.
immunohistochemistry: 1:500 using formalin-fixed, paraffin-embedded human tongue sections and Biotin/ExtrAvidin®-Peroxidase staining system.

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTA2(59)

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This Item
A5228A9357A5691
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

Gene Information

human ... ACTA2(59)

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

Gene Information

human ... ACTC1(70)
mouse ... Actc1(11464)
rat ... Actc1(29275)

Gene Information

mouse ... Acta2(11475)
rat ... Acta2(81633)

species reactivity

rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

species reactivity

rat, human, bovine, chicken, mouse

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

mol wt

antigen ~42 kDa

mol wt

antigen ~42 kDa

mol wt

antigen ~42 kDa

mol wt

antigen ~42 kDa

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General description

Monoclonal Anti-Actin (-Sarcomeric) (mouse IgM isotype) is derived from the hybridoma 5C5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified rabbit striated muscle. Actin is the major cytoskeletal protein in eukaryotic cells.

Immunogen

purified rabbit striated muscle

Application

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal has been used in:
  • indirect immunofluorescence staining
  • immunohistochemistry
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunofluorescence

Biochem/physiol Actions

Actin plays essential roles in a number of cellular processes including cell migration, cytokinesis, vesicle transport and contractile force generation.

Physical form

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Na Tang et al.
Nature communications, 13(1), 7455-7455 (2022-12-03)
Intracellular Ca2+ dysregulation is a key marker in septic cardiac dysfunction; however, regulation of the classic Ca2+ regulatory modules cannot successfully abolish this symptom. Here we show that the knockout of transient receptor potential canonical (TRPC) channel isoforms TRPC1 and
PEDF decreases cardiomyocyte edema during oxygen-glucose deprivation and recovery via inhibiting lactate accumulation and expression of AQP1
Huang B, et al.
International Journal of Molecular Medicine, 43(5), 1979-1990 (2019)
Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles
Miklavc P, et al.
Journal of Cell Science, 128(6), 1193-1203 (2015)
The actin cytoskeleton
Molecular and Cellular Biology, 1979-1990 (2000)
Xinhui Fan et al.
Frontiers in pharmacology, 13, 892643-892643 (2022-07-23)
Diabetes mellitus (DM) often involves cardiovascular complications; however, treatment regimens are limited. ROCK1 (rho-associated coiled-coil containing protein kinase 1) serves as a pathological factor in several diabetic complications. Herein, we aimed to explore the effect of Fasudil (a ROCK1 inhibitor)

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