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T5391

Sigma-Aldrich

apo-Transferrin human

γ-irradiated, powder, BioXtra, suitable for cell culture, ≥98%

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Synonym(s):
Human transferrin, Siderophilin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

sterility

γ-irradiated

product line

BioXtra

assay

≥98%

form

powder

mol wt

76-81 kDa

concentration

~25 mM

technique(s)

cell culture | mammalian: suitable

impurities

HIV and HBsAg, source material tested negative
iron, essentially free

solubility

H2O: 50 mg/mL

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TF(7018)

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General description

apo-Transferrin (aTF) human can be supplemented with iron or used to bind free iron present in media. It can be used to transport iron into mammalian cells.

Application

apo-Transferrin human has been used as a supplement:
  • in Dulbecco′s modified Eagle medium/HamF12 (DMEM/F-12) to culture human adrenocortical carcinoma cells
  • in DMEM/F-12 to culture neural precursor cells from mice
  • in collecting medium NCSU23 to culture preantral follicles from porcine ovaries

Biochem/physiol Actions

apo-Transferrin (aTF) is an endogenous iron chelator and shows protective effects in the host by scavenging free iron and preventing the formation of toxic reactive oxygen species (ROS). It shows a therapeutic effect against thalassemia. After the release of iron, receptor-bound apo-transferrin reaches the cell surface instead of being transported to lysosomes for degradation. The cell surface has a neutral pH that promotes the release of apo-transferrin from its receptor, where it can again circulate and bind additional iron to undergo further rounds of iron delivery to cells.

Other Notes

Non-heme iron-transport protein.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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