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T0665

Sigma-Aldrich

holo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥97%

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Synonym(s):
Siderophilin, iron-saturated
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

biological source

human plasma

description

Iron-saturated

product line

BioReagent

assay

≥97%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

HIV and HBsAg, none detected
endotoxin, tested

solubility

H2O: 50 mg/mL

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... TF(7018)

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1 of 4

This Item
T1147T1283T8158
vibrant-m

T0665

holo-Transferrin human

vibrant-m

T1147

apo-Transferrin human

vibrant-m

T1283

holo-Transferrin bovine

vibrant-m

T8158

Transferrin human

form

powder

form

powder

form

powder

form

powder

biological source

human plasma

biological source

human plasma

biological source

-

biological source

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

impurities

HIV and HBsAg, none detected, endotoxin, tested

impurities

HIV and HBsAg, source material tested negative, ≤0.005% iron

impurities

endotoxin, tested

impurities

endotoxin, tested, hepatitis and HIV antibody, none detected

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.
holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.

Biochem/physiol Actions

Provides iron to cells in a physiologically stable and safe form.

Preparation Note

Heat treated at 60 °C (minimum) for a minimum of 10 hours.

Analysis Note

Iron content by ICP reported on a lot-specific basis.
Purity by agarose gel electrophoresis.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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