MilliporeSigma
All Photos(1)

Documents

T4132

Sigma-Aldrich

holo-Transferrin human

≥98%

Sign Into View Organizational & Contract Pricing

Synonym(s):
Siderophilin, iron-saturated
CAS Number:
EC Number:
MDL number:
NACRES:
NA.61

biological source

human

Quality Level

assay

≥98%

form

powder

Iron content

1100-1600 μg/g

technique(s)

cell culture | mammalian: suitable

impurities

HIV, hepatitis B and hepatitis C, none detected

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... TF(7018)

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
T0665T3309T1408
vibrant-m

T4132

holo-Transferrin human

vibrant-m

T0665

holo-Transferrin human

vibrant-m

T3309

Transferrin human

vibrant-m

T1408

holo-Transferrin bovine

biological source

human

biological source

human plasma

biological source

human

biological source

bovine

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P02787

UniProt accession no.

P02787

UniProt accession no.

P02787

UniProt accession no.

Q29443

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

powder

form

powder

form

powder

form

powder

General description

Transferrin (TF) belongs to the family of bilobal glycoproteins, which bind ferric iron. The homologous N- and C-lobes of the protein have a single iron-binding site present in a deep cleft. The gene encoding TF is localized on human chromosome 3q22.1.

Application

Human holo-transferrin has been used:
  • in colony-forming assays
  • for transferrin uptake and recycling in transfected HEK-293 (human embryonic kidney) cells or bone marrow cells
  • for the preparation of luteinizing and non- luteinizing granulose cells

Biochem/physiol Actions

Studies have shown that co-treatment of breast cancer cells with holo-transferrin, which increases iron levels within cells and derivatives of artemisinin results in increased cell death.
Transferrin (TF) is the iron transport protein in the blood. Iron is transported in the serum by binding to circulating transferrin, which in turn binds to receptors on the cell surface. At the alkaline extracellular pH of 7.4, TF binds one or two ferric ions. The iron-bound TF molecules can bind the dimeric transferrin receptor (TfR). At this pH, iron-free transferrin is not recognized by TfR. This is followed by an endocytotic pathway involving the TfR, where the entire complex is internalized by endocytosis. As the pH reduces in the cell, iron is released from TF. The complex then returns to the cell surface and the apo-TF molecules dissociate from the receptor.

Analysis Note

Purity by agarose gel electrophoresis.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells.
Singh NP
Life Sciences, 21, 49-56 (2001)
EPCR expression marks UM171-expanded CD34+ cord blood stem cells.
Fares I, et al.
Blood, 116(4), blood-b2016 (2017)
Atypical serum transferrin isoform distribution in liver cirrhosis studied by HPLC, capillary electrophoresis and transferrin genotyping.
Arndt T, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 394(1-2), 42-46 (2008)
Hypoxia-inducible factor 1 mediates hypoxia-enhanced synthesis of progesterone during luteinization of granulosa cells.
Yoshioka S, et al.
Journal of Reproduction and Development, 63(1), 75-85 (2017)
Loss of Dynamin 2 GTP ase function results in microcytic anaemia.
Brown F, et al.
British Journal of Haematology, 178(4), 616-628 (2017)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service