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T8944

Sigma-Aldrich

Topoisomerase II α human

(Single band on SDS-PAGE), liquid

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Synonym(s):
Topoisomerase II alpha, TOPO® II, p170
Enzyme Commission number:
MDL number:

Quality Level

form

liquid

mol wt

170 kDa

UniProt accession no.

storage temp.

−70°C

Gene Information

human ... TOP2A(7153)

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This Item
T9069MAB4197HPA026773
vibrant-m

T8944

Topoisomerase II α human

vibrant-m

T9069

Topoisomerase I human

form

liquid

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous glycerol solution

mol wt

170 kDa

mol wt

100 kDa

mol wt

-

mol wt

-

storage temp.

−70°C

storage temp.

−70°C

storage temp.

-

storage temp.

−20°C

UniProt accession no.

P11388

UniProt accession no.

P11387

UniProt accession no.

P11388

UniProt accession no.

P11388

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

General description

Topoisomerase II α (TopoIIα) is a gene product with conserved catalytic activities and it promotes the progression of DNA damage. The α isoform is present in proliferating cells.
Topoisomerase II α is encoded by TOP2A gene, which is located on 17q21.2 in the human chromosome. It is essential for controlling the topological state of DNA by reversibly breaking the DNA strands during replication, recombination and transcription. TOP2A expression increases at late S phase and during G2/M of the cell cycle and decreases after mitosis. Polymorphism with TOP2A causes chromosome instability and tumorigenesis. TOP2A causes tumor proliferation and invasiveness in various cancer types like breast cancer, ovarian cancer and prostate cancer.

Application

Human topoisomerase II α has been used in DNA cleavage assay.
Topoisomerase II α has been used in a study to assess aging processes in the human brain. Topoisomerase II α has also been used in a study to investigate its activity in HIV-1 replication.

Biochem/physiol Actions

Used to study DNA structure and topology.

Unit Definition

One unit will decatenate 0.2 μg of kinetoplast DNA in 30 minutes at 37°C.

Physical form

Buffered aqueous glycerol solution, containing Trizma buffer (pH 7.7). Other components are present in the solution, and lot-specific information on these components may be obtained from Technical Service.

Preparation Note

Recombinant enzyme overexpressed in a proprietary eukaryotic cell line.

Legal Information

TOPO is a registered trademark of Life Technologies

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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J R Spitzner et al.
Nucleic acids research, 16(12), 5533-5556 (1988-06-24)
Topoisomerase II, purified from chicken erythrocytes, was reacted with a large number of different DNA fragments and cleavages were catalogued in the presence and absence of drugs that stabilize the cleavage intermediate. Cleavages were sequenced to derive a consensus for
TOP2A induces malignant character of pancreatic cancer through activating beta-catenin signaling pathway.
Pei YF, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(1), 197-207 (2018)
Topoisomerase 2 alpha: a real predictor of anthracycline efficacy?
Romero A, et al.
Clinical And Translational Oncology, 14(3), 163-168 (2012)
The prognostic significance of topoisomerase II alpha protein in early stage luminal breast cancer.
An X, et al.
BMC Cancer, 18(1), 331-331 (2018)
Raj Kumar Mandraju et al.
Archives of biochemistry and biophysics, 470(1), 27-34 (2007-11-21)
Topoisomerase II alpha (TopoIIalpha) and Topoisomerase II beta (TopoIIbeta) isoforms are different gene products having conserved catalytic activities. The alpha isoform is present in proliferating cell, while beta isoform is predominantly present in non-proliferating cells namely neurons suggesting its role

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