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T9069

Sigma-Aldrich

Topoisomerase I human

buffered aqueous glycerol solution, ≥2 units/μL

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Synonym(s):
DNA Topoisomerase I
Enzyme Commission number:
MDL number:
NACRES:
NA.54

recombinant

expressed in baculovirus

Quality Level

form

buffered aqueous glycerol solution

mol wt

100 kDa

concentration

≥2 units/μL

UniProt accession no.

storage temp.

−70°C

Gene Information

human ... TOP1(7150)

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This Item
T8944WH0007150M1SRP2034
Sigma-Aldrich

T9069

Topoisomerase I human

Sigma-Aldrich

T8944

Topoisomerase II α human

Topo I (N1-197) (NTD) human recombinant, expressed in insect cells, ≥85% (SDS-PAGE)

SRP2034

Topo I (N1-197) (NTD) human

recombinant

expressed in baculovirus

recombinant

-

recombinant

-

recombinant

expressed in insect cells

form

buffered aqueous glycerol solution

form

liquid

form

buffered aqueous solution

form

frozen liquid

concentration

≥2 units/μL

concentration

-

concentration

-

concentration

1000 μg/mL

mol wt

100 kDa

mol wt

170 kDa

mol wt

-

mol wt

~25.3 kDa

UniProt accession no.

P11387

UniProt accession no.

P11388

UniProt accession no.

P11387

UniProt accession no.

P11387

General description

Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.

Application

Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.
Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.

Biochem/physiol Actions

Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer.
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

Unit Definition

One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C.

Physical form

Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Topoisomerase I in human disease pathogenesis and treatments
Li M and Liu Y
Genomics, Proteomics & Bioinformatics, 14(3), 166-171 (2016)
M Lisby et al.
The Journal of biological chemistry, 276(23), 20220-20227 (2001-04-03)
All eukaryotic forms of DNA topoisomerase I contain an extensive and highly charged N-terminal domain. This domain contains several nuclear localization sequences and is essential for in vivo function of the enzyme. However, so far no direct function of the
Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA
Redinbo MR, et al.
Biochemistry, 39(23), 6832-6840 (2000)
Binding and dissociation of human topoisomerase I with hairpin-loop RNAs: implications for the regulation of HIV-1 replication
Takahashi H, Sawa H, Hasegawa H,
Biochemical and Biophysical Research Communications, 297(3), 593-599 (2012)
Molecular Networking Reveals Two Distinct Chemotypes in Pyrroloiminoquinone-Producing Tsitsikamma favus Sponges
Kalinski JCJ, et al.
Marine drugs, 17(1), 60-60 (2019)

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