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ZRB1008

Sigma-Aldrich

Anti-GluR2 Antibody, clone 1K4, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
AMPA-selective glutamate receptor 2, GluA2, GluR-2, GluR-B, GluR-K2, Glutamate receptor 2, Glutamate receptor ionotropic AMPA 2

biological source

rabbit (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1K4, recombinant monoclonal
monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 98.69 kDa
observed mol wt ~100 kDa

species reactivity

mouse, human, rat

species reactivity (predicted by homology)

bovine

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: suitable using 1:100
immunofluorescence: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1,000
western blot: suitable using 1:1,000

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1K4 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glutamate receptor 2 (GluR2). It targets an epitope with in 16 amino acids from the C-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal cytoplasmic domain of rat Glutamate receptor 2.

Application

Anti-GluR2, clone 1K4 ZooMAb, Cat. No. ZRB1008, is a recombinant Rabbit Monoclonal Antibody that specifically targets Glutamate receptor 2 and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GluR2 in rat and mouse brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected GluR2 in rat brain and human brain tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GluR2 in human brain and rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GluR2 in Rat E18 cortical neurons.

Target description

Glutamate receptor 2 (UniProt P19491; also known as AMPA-selective glutamate receptor 2, GluA2, GluR-2, GluR-B, GluR-K2, GluR2, Glutamate receptor ionotropic, AMPA 2) is encoded by the Gria2 (also known as Glur2) gene (Gene ID: 29627) in rat species. Glutamate receptors are synaptic receptors located primarily on the membranes of neuronal cells. These receptors play important roles in neural communication, memory formation, learning, and regulation by mediating glutamate-dependent postsynaptic excitation. Glutamate receptors are divided into two types, ionotropic glutamate receptors (iGluRs) and metabotropic glutamate receptors (mGluRs). iGluRs form ion channel pores upon glutamate binding, while mGluRs indirectly activate ion channels on the plasma membrane via G protein-coupled signaling cascade. Although all iGluRs and mGluRs bind glutamate, they do exhibit different binding activities toward chemical ligands, such as NMDA, kainate, AMPA, L-AP4, ACPD, L-QA, and such distinction is used to divide these receptors further into subtypes. GluR-2 is an AMPA type iGluR. It is a multi-pass membrane protein that is initially produced with an N -terminal signal peptide sequence (aa. 1-21), the removal of which yields the mature receptor. GluR-2 is palmitoylated in resting state and is depalmitoylated upon glutamate stimulation. Palmitoylation at cysteine 610 leads to its Golgi retention and decreased cell surface expression. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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