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HPLC Analysis of Leucine Enantiomers on Astec® CHIROBIOTIC® T (25 °C)

application for HPLC

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HPLC: suitable

test parameters

column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] water; [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 205 nm
injection: 10 μL
sample: 300 μg /mL in 30:70 water:methanol


application for HPLC


life science and biopharma

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC



    L-Leucine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
    D-Leucine, ≥98% (TLC)

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