Skip to Content
MilliporeSigma
  • Rab14 regulates maturation of macrophage phagosomes containing the fungal pathogen Candida albicans and outcome of the host-pathogen interaction.

Rab14 regulates maturation of macrophage phagosomes containing the fungal pathogen Candida albicans and outcome of the host-pathogen interaction.

Infection and immunity (2015-02-04)
Blessing Okai, Natalie Lyall, Neil A R Gow, Judith M Bain, Lars-Peter Erwig
ABSTRACT

Avoidance of innate immune defense is an important mechanism contributing to the pathogenicity of microorganisms. The fungal pathogen Candida albicans undergoes morphogenetic switching from the yeast to the filamentous hyphal form following phagocytosis by macrophages, facilitating its escape from the phagosome, which can result in host cell lysis. We show that the intracellular host trafficking GTPase Rab14 plays an important role in protecting macrophages from lysis mediated by C. albicans hyphae. Live-cell imaging of macrophages expressing green fluorescent protein (GFP)-tagged Rab14 or dominant negative Rab14, or with small interfering RNA (siRNA)-mediated knockdown of Rab14, revealed the temporal dynamics of this protein and its influence on the maturation of macrophage phagosomes following the engulfment of C. albicans cells. Phagosomes containing live C. albicans cells became transiently Rab14 positive within 2 min following engulfment. The duration of Rab14 retention on phagosomes was prolonged for hyphal cargo and was directly proportional to hyphal length. Interference with endogenous Rab14 did not affect the migration of macrophages toward C. albicans cells, the rate of engulfment, the overall uptake of fungal cells, or early phagosome processing. However, Rab14 depletion delayed the acquisition of the late phagosome maturation markers LAMP1 and lysosomal cathepsin, indicating delayed formation of a fully bioactive lysosome. This was associated with a significant increase in the level of macrophage killing by C. albicans. Therefore, Rab14 activity promotes phagosome maturation during C. albicans infection but is dysregulated on the phagosome in the presence of the invasive hyphal form, which favors fungal survival and escape.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
BIS-TRIS, BioUltra, ≥99.0% (NT)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Supelco
Water, for HPCE, for luminescence, suitable for UV/Vis spectroscopy
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Water, tested according to Ph. Eur.
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Water, for cell biology, sterile ultrafiltered
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Water, PCR Reagent
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
BIS-TRIS, BioXtra, ≥98.0% (titration)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Water, for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
BIS-TRIS, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Water, Nuclease-Free Water, for Molecular Biology
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
BIS-TRIS, ≥98.0% (titration)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Water, sterile-filtered, BioReagent, suitable for cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Adenine, BioReagent, suitable for plant cell culture, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Adenine, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Aphidicolin from Nigrospora sphaerica, ≥98% (HPLC), powder
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
6-Carboxyfluorescein, ≥96% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Supelco
Density Standard 998 kg/m3, H&D Fitzgerald Ltd. Quality
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Supelco
Aphidicolin, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Supelco
Water, for TOC analysis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Supelco
Water, suitable for ion chromatography
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Adenine, BioReagent, suitable for cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Water, for molecular biology, sterile filtered
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
E-Toxate Water, endotoxin, free
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Supelco
Water, ACS reagent, for ultratrace analysis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Adenine, European Pharmacopoeia (EP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
SAFC
BIS-TRIS
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Pure Water Density Standard, UKAS ISO/IEC17025 and ISO Guide 34 certified, density: 0.9982 g/mL at 20 °C, density: 0.9970 g/mL at 25 °C
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Pure Water Density Standard, UKAS ISO/IEC17025 and ISO Guide 34 certified, density: 0.9982 g/mL at 20 °C, density: 0.9970 g/mL at 25 °C
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Supelco
Adenine, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
Sigma-Aldrich
Water, BioPerformance Certified
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00
SAFC
BIS-TRIS
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
Estimated to ship on February 20, 2025
$444.00