Skip to Content
MilliporeSigma
  • A new approach for automated liquid-liquid extraction in a sequential injection manifold.

A new approach for automated liquid-liquid extraction in a sequential injection manifold.

Analytical and bioanalytical chemistry (2014-11-06)
Irene Delgado-Blanca, Eulogio J Llorent-Martínez, Antonio Ruiz-Medina, Pilar Ortega-Barrales
ABSTRACT

A new approach for the implementation of liquid-liquid extraction in a sequential injection manifold is presented. The manifold consists of two syringe pumps and one multi-position valve. The use of a 30 % MeOH/H2O (v/v) solution as the carrier, with isobutanol as the extractant, made it possible to avoid the problems associated with the different affinities of the organic and aqueous phases for Teflon tubing, and the formation of bubbles. The suitability of the proposed method was demonstrated by the determination of thiamine in pharmaceutical preparations and dietary supplements. Detection and quantification limits were estimated as 9 and 30 μg L(-1), respectively. The repeatability was lower than 3 % and the intermediate precision (3 d) was lower than 7 %. The sample throughput was 14 samples per h. The results were in agreement with a high-performance liquid chromatography-electrospray/mass spectrometry reference method.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chloroform, ACS reagent, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
4 x 4 L
1 Available to ship on January 09, 2025
Details...
$1,020.00
Supelco
Methanol, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 mL
1 Available to ship on January 09, 2025
Details...
$48.60
10 mL
1 Available to ship on January 09, 2025
Details...
$96.50
Supelco
Chloroform, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 mL
1 Available to ship on January 09, 2025
Details...
$165.00
Supelco
Thiamine Hydrochloride (B1), analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
1 Available to ship on January 09, 2025
Details...
$61.00
1 L
1 Available to ship on January 09, 2025
Details...
$115.00
2 L
1 Available to ship on January 09, 2025
Details...
$181.00
6 x 1 L
1 Available to ship on January 09, 2025
Details...
$556.00
12 x 100 mL
1 Available to ship on January 08, 2025
Details...
$594.00
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, 99.93%
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Chloroform, ACS reagent, ≥99.8%, contains amylenes as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
4 x 4 L
1 Estimated to ship on March 09, 2025
$847.00
Sigma-Aldrich
Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
1 Available to ship on January 09, 2025
Details...
$61.50
1 L
1 Available to ship on January 09, 2025
Details...
$114.00
2 L
1 Available to ship on January 09, 2025
Details...
$157.00
12 x 100 mL
1 Available to ship on January 09, 2025
Details...
$590.00
$295.00
Sigma-Aldrich
2-Methyl-1-propanol, BioUltra, for molecular biology, ≥99.5% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
2-Methyl-1-propanol, anhydrous, 99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 L
1 Available to ship on January 09, 2025
Details...
$100.00
2 L
1 Available to ship on January 09, 2025
Details...
$152.00
4 x 2 L
1 Available to ship on January 09, 2025
Details...
$384.00
6 x 1 L
1 Available to ship on January 09, 2025
Details...
$480.00
12 x 100 mL
1 Available to ship on January 09, 2025
Details...
$482.00
18 L
1 Available to ship on January 08, 2025
Details...
This made-to-order SKU is packaged in a Returnable Container and may require a deposit. To learn more, contact ReCycler@milliporesigma.com.
$846.00
Supelco
Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
4 L
1 Available to ship on January 09, 2025
Details...
$311.00
4 x 4 L
Only 54 left in stock
Details...
$1,050.00
Sigma-Aldrich
Thiamine hydrochloride, ≥98%, FCC, FG
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 Available to ship on January 09, 2025
Details...
$54.60
1 kg
1 Available to ship on January 09, 2025
Details...
$169.00
5 kg
1 Available to ship on January 09, 2025
Details...
$789.00
10 kg
1 Available to ship on January 09, 2025
Details...
$1,470.00
Sigma-Aldrich
Thiamine hydrochloride, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 g
1 Available to ship on January 09, 2025
Details...
$77.70
250 g
1 Available to ship on January 09, 2025
Details...
$551.00
$484.88
Sigma-Aldrich
Thiamine hydrochloride, reagent grade, ≥99% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
1 Available to ship on January 09, 2025
Details...
$21.00
10 g
1 Available to ship on January 09, 2025
Details...
$39.80
25 g
1 Available to ship on January 09, 2025
Details...
$65.30
100 g
1 Available to ship on January 09, 2025
Details...
$92.60
250 g
1 Available to ship on January 09, 2025
Details...
$206.00
1 kg
1 Available to ship on January 09, 2025
Details...
$556.00
Sigma-Aldrich
Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 vials
1 Estimated to ship on March 09, 2025
$131.00
Sigma-Aldrich
Thiamine hydrochloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 g
1 Available to ship on January 09, 2025
Details...
$68.80
100 g
1 Available to ship on January 09, 2025
Details...
$141.00
Supelco
2-Methyl-1-propanol, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 mL
1 Available to ship on January 09, 2025
Details...
$43.80
5 mL
1 Estimated to ship on March 09, 2025
$176.00
10 mL
1 Available to ship on January 09, 2025
Details...
$313.00
Sigma-Aldrich
Thiamine hydrochloride, tested according to Ph. Eur.
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 g
1 Available to ship on January 09, 2025
Details...
$84.30
Sigma-Aldrich
Methanol, NMR reference standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 pkg
1 Estimated to ship on March 09, 2025
$188.00
1 pkg
1 Estimated to ship on March 09, 2025
$193.00
$38.60
Supelco
Thiamine hydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Available to ship on January 09, 2025
Details...
$109.00
Supelco
Heptafluorobutyric acid solution, 0.5 M in H2O, LiChropur, suitable for ion pair chromatography
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
1 Available to ship on January 09, 2025
Details...
$827.00
Sigma-Aldrich
Methanol, SAJ special grade
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, suitable for HPLC
Pricing and availability is not currently available.
Sigma-Aldrich
2-Methyl-1-propanol, JIS special grade, ≥99.0%
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, SAJ first grade, ≥99.0%, contains 0.4-0.8% ethanol
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, JIS 300, ≥99.8%, for residue analysis
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, JIS 300, ≥99.0%, for residue analysis
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, JIS special grade, ≥99.0%
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, SAJ super special grade, ≥99.0%
Pricing and availability is not currently available.