- In situ formation of H2O2 for P450 peroxygenases.
In situ formation of H2O2 for P450 peroxygenases.
Bioorganic & medicinal chemistry (2014-07-06)
Caroline E Paul, Ekaterina Churakova, Elmer Maurits, Marco Girhard, Vlada B Urlacher, Frank Hollmann
PMID24984939
ABSTRACT
An in situ H2O2 generation approach to promote P450 peroxygenases catalysis was developed through the use of the nicotinamide cofactor analogue 1-benzyl-1,4-dihydronicotinamide (BNAH) and flavin mononucleotide (FMN). Final productivity could be enhanced due to higher enzyme stability at low H2O2 concentrations. The H2O2 generation represented the rate-limiting step, however it could be easily controlled by varying both FMN and BNAH concentrations. Further characterization can result in an optimized ratio of FMN/BNAH/O2/biocatalyst enabling high reaction rates while minimizing H2O2-related inactivation of the enzyme.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Trizma® base, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Trometamol, European Pharmacopoeia (EP) Reference Standard
Lauric acid, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Chlorotrimethylsilane, produced by Wacker Chemie AG, Burghausen, Germany, ≥99.0% (GC)