Skip to Content
MilliporeSigma
  • Embryonic catalase protects against ethanol embryopathies in acatalasemic mice and transgenic human catalase-expressing mice in embryo culture.

Embryonic catalase protects against ethanol embryopathies in acatalasemic mice and transgenic human catalase-expressing mice in embryo culture.

Toxicology and applied pharmacology (2015-06-16)
Lutfiya Miller-Pinsler, Peter G Wells
ABSTRACT

Reactive oxygen species (ROS) have been implicated in the mechanism of ethanol (EtOH) teratogenicity, but the protective role of the embryonic antioxidative enzyme catalase is unclear, as embryonic activity is only about 5% of maternal levels. We addressed this question in a whole embryo culture model. C57BL/6 mouse embryos expressing human catalase (hCat) or their wild-type (C57BL/6 WT) controls, and C3Ga.Cg-Cat(b)/J catalase-deficient, acatalasemic (aCat) mouse embryos or their wild-type C3HeB/FeJ (C3H WT) controls, were explanted on gestational day (GD) 9 (plug=GD 1), exposed for 24h to 2 or 4mg/mL EtOH or vehicle, and evaluated for functional and morphological changes. hCat and C57BL/6 WT vehicle-exposed embryos developed normally, while EtOH was embryopathic in C57BL/6 WT embryos, evidenced by decreases in anterior neuropore closure, somites developed, turning and head length, whereas hCat embryos were protected (p<0.001). Maternal pretreatment of C57BL/6 WT dams with 50kU/kg PEG-catalase (PEG-cat) 8h prior to embryo culture, which increases embryonic catalase activity, blocked all EtOH embryopathies (p<0.001). Vehicle-exposed aCat mouse embryos had lower yolk sac diameters compared to WT controls, suggesting that endogenous ROS are embryopathic. EtOH was more embryopathic in aCat embryos than WT controls, evidenced by reduced head length and somite development (p<0.01), and trends for reduced anterior neuropore closure, turning and crown-rump length. Maternal pretreatment of aCat dams with PEG-Cat blocked all EtOH embryopathies (p<0.05). These data suggest that embryonic catalase is a determinant of risk for EtOH embryopathies.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Silica, fumed, powder, 0.2-0.3 μm avg. part. size (aggregate)
Sigma-Aldrich
HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
Sigma-Aldrich
HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)
Sigma-Aldrich
HEPES, ≥99.5% (titration)
Sigma-Aldrich
HEPES, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)
Sigma-Aldrich
Silica, fumed, powder
Sigma-Aldrich
Silica, mesostructured, MSU-F (cellular foam)
Sigma-Aldrich
Silicon dioxide, granular, ≥99.9%
Sigma-Aldrich
Silicon dioxide, fused (granular), 4-20 mesh, 99.9% trace metals basis
Supelco
Glass spheres
Sigma-Aldrich
Silica
Supelco
Silica, 99.8%
Sigma-Aldrich
LUDOX® SM colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
Silicon dioxide, −325 mesh, 99.5% trace metals basis
Sigma-Aldrich
LUDOX® HS-30 colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® LS colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® TM-40 colloidal silica, 40 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® CL colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
HEPES, BioUltra, for molecular biology, ≥99.5% (T)
Sigma-Aldrich
Silicon dioxide, JIS special grade
Sigma-Aldrich
Silica, nanopowder, 99.8% trace metals basis
Sigma-Aldrich
Silicon dioxide, nanopowder (spherical, porous), 5-20 nm particle size (TEM), 99.5% trace metals basis
Sigma-Aldrich
Silicon dioxide, nanopowder, 10-20 nm particle size (BET), 99.5% trace metals basis
Sigma-Aldrich
Silica, mesostructured, MCM-41 type (hexagonal)
Sigma-Aldrich
Magnesium chloride solution, 0.1 M
Sigma-Aldrich
Silicon dioxide, single crystal substrate, optical grade, 99.99% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm
Sigma-Aldrich
Silica, nanoparticles, mesoporous, 200 nm particle size, pore size 4 nm
SAFC
HEPES
SAFC
HEPES
Sigma-Aldrich
Magnesium chloride solution, BioUltra, for molecular biology, ~1 M in H2O