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保管温度
−20°C
詳細
The Dione MicroMap Kit provides a method for small molecule intracellular target identification via photocatalytic proximity labeling. The kit contains a catalyst that can be coupled to an acid on almost any small-molecule ligand. The subsequent ligand-Iridium conjugate can be used to perform multiple target identification experiments. The following kit provides enough Ir-catalyst to synthesize 20 μMoles of iridium–ligand conjugate and enough Diazirine-PEG3-Biotin to perform 10 proteomics experiments.
The kit provides a step-by-step procedure for conjugating your ligand of choice to a photocatalyst that localizes light-mediated crosslinking to the ligands target. The ligand–iridium conjugate can then be incubated with live cells along with a biotinylated diazirine reagent. Analogous to a satellite, the photocatalyst localizes carbene generation from the diazirine only in the direct vicinity and thus covalently modifies the target protein in a catalytic manner, amplifying the signal for downstream analysis.
To link to an amine on a small molecule (instead of an acid), the Hyas Kit is available.
Photoreactors commercially available:
The kit provides a step-by-step procedure for conjugating your ligand of choice to a photocatalyst that localizes light-mediated crosslinking to the ligands target. The ligand–iridium conjugate can then be incubated with live cells along with a biotinylated diazirine reagent. Analogous to a satellite, the photocatalyst localizes carbene generation from the diazirine only in the direct vicinity and thus covalently modifies the target protein in a catalytic manner, amplifying the signal for downstream analysis.
To link to an amine on a small molecule (instead of an acid), the Hyas Kit is available.
Photoreactors commercially available:
- Penn PhD Photoreactor M2 (Z744035)
- Penn PhD Photoreactor M2 365nm Light Source (Z744031)
- LightOX PhotoReact 365 (Z744061)
Read more about: - Target identification with HyasKit and DioneKit in our Technology Spotlight
- Small molecule photocatalysis enables drug target identification via energy transfer
アプリケーション
Proximity protein labeling, protein-protein interactions, photoaffinity labeling, proteomics
生物化学的／生理学的作用
Kit of components packaged in individual vials for enabling photoinduced proximity labeling. The kit contains an Ir-catalyst to couple to an acid on a small molecule ligand. Kit contains Ir-catalyst and Diazirine PEG3 Biotin to perform 10 proteomics experiments. To link to an amine on a small molecule, the Dione Kit is available.
保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
DIONEKIT-A:
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資料
In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.
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