おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
26-2F, monoclonal
化学種の反応性
human
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
inhibition assay: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ANG(283)
詳細
Angiogenin (Ang) is a non-glycosylated polypeptide, 123 amino acids in length, with a molecular mass of 14 kDa. It is secreted by tumor cells and is a potent inducer of neovascularization. Angiogenin is produced by a variety of cell types including vascular endothelial cells and smooth muscle cells, fibroblasts, tumor colonic epithelium, normal peripheral blood lymphocytes, lung and colonic epithelial tumor cell lines, and primary gastrointestinal adenocarcinomas. It specifically binds to endothelial cells and elicits second messenger systems. Angiogenin is present in human follicular fluid, and its production is up-regulated by human chorionic gonadotropin and hypoxic conditions. Angiogenin shows a high degree of homology with known ribonucleases such as pancreatic ribonuclease A, and the capacity of angiogenin to induce blood vessel growth is dependent on its ribo-nucleolytic activity. Angiogenin is thought to be involved in the development of solid tumors; angiogenin antagonists are capable of inhibiting tumor growth. Angiogenin is endocytosed by subconfluent endothelial cells and translocated to the nucleus, where it accumulates in the nucleolus. Serum angiogenin concentration is increased in serum of colorectal cancer patients, and correlates with cancer progression. The cytoskeletal protein α-actinin-2 binds to angiogenin, which might be a novel interface target for anti-angiogenesis and anti-tumor therapy.
免疫原
Purified Human Angiogenin.
アプリケーション
Research Category
アポトーシス及び癌
アポトーシス及び癌
Research Sub Category
アポトーシス-追加
アポトーシス-追加
Inhibition Assay: A representative lot of this antibody blocked cell-free protein synthesis by angiogenin (Fett et al., (1994) Biochemistry. 33(18):5421-5427).
Inhibition Assay: A representative lot of this antibody inhibited ribonucleolytic activity (Piccoli et al., (1998) Proc. Natl. Acad. Sci. 95:4579–4583).
Immunohistochemistry Analysis: A representative lot of this antibody detected Angiogenin in Neovessels of cancer tissue (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Immunocytochemistry Analysis: A representative lot of this antibody detected Angiogenin in HeLa and Huvec cells (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Inhibition Assay: A representative lot of this antibody inhibited ribonucleolytic activity (Piccoli et al., (1998) Proc. Natl. Acad. Sci. 95:4579–4583).
Immunohistochemistry Analysis: A representative lot of this antibody detected Angiogenin in Neovessels of cancer tissue (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Immunocytochemistry Analysis: A representative lot of this antibody detected Angiogenin in HeLa and Huvec cells (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
This Anti-Angiogenin Antibody, clone 26–2F (Azide Free) is validated for use in Immunocytochemistry and Inhibition and Immunohistochemistry for the detection of Angiogenin.
品質
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 4 µg/mL of this antibody detected Angiogenin in Angiogenin-treated and untreated HeLa Cells. HeLa cells that were treated with Angiogenin up-took the protein and translocated it to the nucleus. Untreated HeLa cells did not exhibit any Angiogenin protein signal.
Immunocytochemistry Analysis: A 4 µg/mL of this antibody detected Angiogenin in Angiogenin-treated and untreated HeLa Cells. HeLa cells that were treated with Angiogenin up-took the protein and translocated it to the nucleus. Untreated HeLa cells did not exhibit any Angiogenin protein signal.
ターゲットの説明
16 kDa calculated
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABC166:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Aleksej Drino et al.
Nucleic acids research, 51(3), 1326-1352 (2023-02-01)
Stress-induced tRNA fragmentation upon environmental insult is a conserved cellular process catalysed by endonucleolytic activities targeting mature tRNAs. The resulting tRNA-derived small RNAs (tsRNAs) have been implicated in various biological processes that impact cell-to-cell signalling, cell survival as well as
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）