33247

尿素

puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%

• 別名: カルバミド, カルボニルジアミド
• 化学式: NH₂CONH₂
• CAS番号: 57-13-6
• 分子量: 60.06
• Beilstein: 635724
• EC Number: 200-315-5
• MDL番号: MFCD00008022
• UNSPSCコード: 12352100
• PubChem Substance ID: 57648752
• NACRES: NA.21

特性

• グレード: ACS reagent; puriss. p.a.
• 品質水準: 200
• 認証: reag. Ph. Eur.
• アッセイ: ≥99%; 99-101% (calc. on dry substance)
• 形状: solid
• 不純物: alkalic reac. substances, complies; ≤0.0005% heavy metals (as Pb); ≤0.05% ammonium (NH₄); ≤0.1% biuret
• 強熱残分: ≤0.01% (as SO₄)
• 損失: ≤1.0% loss on drying, 105°C, 1 h
• mp: 132-135 °C (lit.); 132-135 °C
• 溶解性: H₂O: soluble 480 g/L at 20 °C
• 密度: 1.335 g/mL at 25 °C (lit.)
• 微量陰イオン: chloride (Cl^-): ≤5 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• 微量陽イオン: Fe: ≤2 mg/kg
• 適合性: in accordance for appearance of solution; in accordance for identity
• 官能基: amine
• SMILES記法: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI Key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• 遺伝子情報: human ... CA1(759) , CA2(760) ; rat ... Ppm1a(24666)

詳細

詳細

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.

Urea is the most commonly used slow release nitrogen fertilizer.

アプリケーション

Urea has been used in the following studies:

• Synthesis of SnO₂ nanoparticles.
• Synthesis of urethanes, ureides and semicarbazides.
• Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain.
• Protein electrophoretic studies.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

タンパク質の変性剤として、また不溶性タンパク質や変性タンパク質に対する穏やかな可溶化剤として用いられています。6 M塩酸グアニジンですでに変性された封入体などのサンプルからタンパク質を復元する用途に適しています。変性タンパク質を天然型または活性型にリフォールディングさせる際に、塩酸グアニジンやジチオスレイトール(DTT)とともに使用することができます。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)