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Merck
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主要文書

5006

Sigma-Aldrich

Bovine Collagen Type I & III

from bovine hide, powder, suitable for cell culture, PureCol™

別名:

Collagen Type I, PureCol Collagen

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About This Item

EC Number:
232-697-4
UNSPSCコード:
12352202
NACRES:
NA.75

product name

PureCol™, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

品質水準

100

無菌性

sterile-filtered

アッセイ

99.9%

形状

lyophilized

包装

pkg of 15 mg

テクニック

cell culture | mammalian: suitable

結合特異性

Peptide Source: Elastin

Peptide Source: Fibrinogen

輸送温度

wet ice

保管温度

−20°C

関連するカテゴリー

詳細

PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

アプリケーション

PureCol™ has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

調製ノート

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

法的情報

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

保管分類コード

11 - Combustible Solids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

試験成績書（COA）

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資料

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

細胞外基質タンパク質と培養細胞の最適化ツール

ラミニン、コラーゲン、フィブロネクチンなどの細胞外基質タンパク質は細胞培養の際の細胞接着基質として使用されます。

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

3D細胞培養ツールおよび技術

3D細胞培養の概要2D細胞培養と3D細胞培養の比較、3D細胞培養の利点、3D細胞培養技術について紹介します。

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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