A1559

モノクロナール抗ビオチン-アガロース マウス宿主抗体

clone BN-34, purified immunoglobulin, PBS suspension

• 別名: モノクロナール抗ビオチン
• UNSPSCコード: 12352203
• NACRES: NA.46
• 結合体: agarose conjugate
• application: ELISA; IHC; IP; WB
• クローン: BN-34, monoclonal
• テクニック: ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 8

特性

• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in mouse cell line
• 結合体: agarose conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: BN-34, monoclonal
• フォーム: PBS suspension
• 標識化の程度: 2 mg antibody per mL resin
• テクニック: ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1
• キャパシティ: 0.15-0.3 μmol/mL, agarose binding capacity (biotin-ligand conjugate)
• アプリケーション: research pathology
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

競合ELISAを用いた場合、本抗体は遊離ビオチンおよびビオサイチンを認識します。間接ELISAまたは免疫組織化学染色を用いた場合、本抗体は、さまざまな免疫グロブリンに結合したビオチンも認識します。特異性は、マルチウェルプレートにコーティングされたヒトおよびウサギの抗原に対するビオチン化ヤギ抗体を使用して評価されました。

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.

免疫原

ビオチン-KLH結合体

アプリケーション

本抗体は、増幅技術、ELISA、免疫ブロッティング、in situ核酸ハイブリダイゼーション、フローサイトメトリー、画像分析、または共焦点顕微鏡法に使用される場合があります。
一部のアプリケーションでは、アビジンによるビオチン化プローブの局在化により、バックグラウンドレベルが高くなります。非特異的結合を減らすために、抗ビオチン試薬がアビジンの代わりに使用される場合があります。

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Monoclonal Anti-Biotin-Agarose antibody produced in mouse has been used in:

• pulldown assay
• coimmunoprecipitation 
• chromatin immunoprecipitation
• purification of biotinylated compounds and biotin-containing compounds

Mouse monoclonal anti-biotin antibody was used to identify by western blot S-nitrosylated proteins that had been labeled with a biotin moeity based on the biotin-switch method. No reactivity was visible on the blots when the biotin switch method was inhibited by treatment with 100 mM dithiothreitol showing that this antibody shows no unspecific cross reactivity. The mouse monoclonal anti-biotin antibody does cross react with Arabidopsis plant by western blot, no cross reactivity with unbiotinylated proteins.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

物理的形状

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

調製ノート

精製抗体を臭化シアン活性化アガロ-スとカップリングして調製しています。

その他情報

Polyclonal anti-biotin antibodies were shown to react with in vivo biotinylated proteins but not monoclonal anti-biotin antibodies.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable