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Merck

A4327

Sigma-Aldrich

アルブミン ヒト血清由来

lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis)

別名:

HSA

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About This Item

CAS番号:
EC Number:
MDL番号:
UNSPSCコード:
12352202
NACRES:
NA.25

由来生物

human

品質水準

アッセイ

≥96% (agarose gel electrophoresis)

フォーム

lyophilized powder

分子量

monomer calculated mol wt 66478 Da

テクニック

tissue culture: suitable

不純物

HIV I and HIVII, HCV and HBsAg, tested negative

溶解性

H2O: soluble 50 mg/mL

UniProtアクセッション番号

その他の活性

Alkaline phosphatase ≤0.001 units/mg protein
Peroxidase ≤0.001 units/mg protein
Protease ≤0.0001 units/mg protein

保管温度

2-8°C

SMILES記法

FC(F)(F)C(F)(F)C(F)(F)F

InChI

1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11

InChI Key

QYSGYZVSCZSLHT-UHFFFAOYSA-N

遺伝子情報

human ... ALB(213)

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詳細

Albumin is the most copious protein in blood plasma. Produced in the liver, serum albumin serves as a carrier protein for biomolecules like as fatty acids, amino acids, and steroids.

This human serum albumin (HSA) product has been specially tested and evaluated to be low in the following properties:
  • Essentially protease-free / low protease content
  • Low alkaline phosphatase content
  • Low peroxidase content

Serum albumin is used in various applications related to molecular biology, notably as an inert protein stabilizer for stock solutions of enzymes and proteins of interest. In such applications, it is thus important for the HSA to be protease free, to maintain the integrity of the protein or enzyme of interest.

Protease-free HSA is especially useful for applications like:
  • Enzyme assays
  • Protein-based assays
  • Protease-sensitive techniques such as:
(a) Enzyme immunoassay (EIA)
(b) Nucleic acid hybridization
(c) Radioimmunoassay (RIA)

アプリケーション

Albumin was used to test its effect on the in vitro bactericidal activity of cefditoren against penicillin-resistant Streptococcus pneumonia. It has been clinically used in serious and often life-threatening conditions, such as shock and blood loss due to trauma, burns, and surgery.
It was used to test the effect of non-enzymatic glycation on the unfolding of human serum albumin. It was also used to test the two distinct pathways used by intestinal epithelial cells for HLA class II antigen processing.

生物化学的/生理学的作用

血清アルブミンはステロイド、脂肪酸、甲状腺ホルモンの担体タンパク質として機能し、血液のコロイド浸透圧の調節にも不可欠です。またアルブミンは、薬物(イブプロフェン、ワルファリンなど)を始めとした外因性物質に結合し、薬物動態に強く影響を及ぼします。アルブミンの酸化還元状態の変化につながる酸化ストレスは、その生理機能に様々な影響を及ぼします。

特徴および利点

  • Low protease activity / essentially protease-free (≤ 0.0001 units/mg protein)
  • Low alkaline phosphatase activity (≤ 0.001 units/mg protein)
  • Low peroxidase activity (≤ 0.001 units/mg protein)

調製ノート

A1653から調製されています。

その他情報

より詳細な情報につきましては、human serum albuminをご覧ください。

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

A4327-500MG:
A4327-1G:
A4327-VAR:
A4327-BULK:


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試験成績書(COA)

Lot/Batch Number

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以前この製品を購入いただいたことがある場合

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文書ライブラリにアクセスする

Kuldip S Sidhu et al.
Current protocols in stem cell biology, Chapter 1, Unit 1A-Unit 1A (2008-09-05)
The pluripotent nature of human embryonic stem cells (hESC) makes them very attractive as a source of various cell types that could be used therapeutically in regenerative medicine. However, eliminating all sources of contamination, animal-derived or human cell-derived, during hESC
Deepak Kumar et al.
Talanta, 224, 121906-121906 (2021-01-01)
Human serum albumin (HSA) has pseudoesterase activity. So far on gel specific detection of such property of HSA is never reported. Moreover, protein binding dyes are non-specific for albumin. However, many of such dyes are used for HSA detection. So
Natalia Malachowa et al.
mBio, 10(6) (2019-12-19)
Klebsiella pneumoniae is a human gut communal organism and notorious opportunistic pathogen. The relative high burden of asymptomatic colonization by K. pneumoniae is often compounded by multidrug resistance-a potential problem for individuals with significant comorbidities or other risk factors for
R M Hershberg et al.
The Journal of clinical investigation, 100(1), 204-215 (1997-07-01)
Intestinal epithelial cells express a low level of HLA class II molecules constitutively, with elevated levels seen in the setting of mucosal inflammation including inflammatory bowel disease. The ability of intestinal epithelial cells to act as antigen presenting cells for
Karin Löw et al.
FEBS letters, 594(17), 2840-2866 (2020-06-09)
Bioactive peptide drugs hold promise for therapeutic application due to their high potency and selectivity but display short plasma half-life. Examination of selected naturally occurring peptide hormones derived from proteolytic cleavage of the proopiomelanocortin (POMC) precursor lead to the identification

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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