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Merck

A8275

Sigma-Aldrich

抗ウサギIgG (全分子)-ペルオキシダーゼ ヤギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

別名:

Goat Anti-Rabbit IgG (whole molecule)–HRP

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.46

由来生物

goat

品質水準

結合体

peroxidase conjugate

抗体製品の状態

IgG fraction of antiserum

抗体製品タイプ

secondary antibodies

クローン

polyclonal

形状

buffered aqueous solution

テクニック

direct ELISA: 1:10,000

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

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詳細

IgG, a monomer, is an antibody circulating in blood and can assist in phagocytic destruction of foreign microorganism. It plays an important role in Antibody dependent cell mediated cytotoxicity and is also associated with type II and type III hypersensitivity. Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody can be used in pAb-ELISA. Rabbit anti-IgG antibody coupled with peroxidase (1:2500 dilution in TBS, 0.5% BSA) can be used in western blots. This antibody is also used as detector in indirect ELISA for saxitoxin analysis. Goat anti-rabbit IgG Peroxidase conjugate reacted specifically with all rabbit immunoglobulin.

免疫原

Purified rabbit IgG.

アプリケーション

二次抗体としてHRP抱合ヤギ抗ウサギIgGを用い、C33A細胞溶解物の共免疫沈降分析およびウエスタンブロット分析が行われました。
Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody is used in western blotting, Indirect ELISA, Immunoblotting and Immunodetection.
Immunohistochemistry was performed on frozen sections (10um) of mouse intestine, liver, and spleen using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Prior to incubation with the secondary, sections were treated with a mixture of MeOH/hydrogen peroxide 30% to block endogenouse peroxidases.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

調製ノート

Wilson, M.B., and Nakane, P.K., Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978) に示された過ヨウ素酸塩法を用いて調製しました。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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ピクトグラム

Exclamation markEnvironment

シグナルワード

Warning

危険有害性情報

危険有害性の分類

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

A8275-2ML:4548173275833
A8275PROC:
A8275-1ML:4548173187396
A8275-VAR:
A8275-.5ML:4548173187389
A8275-BULK:


試験成績書(COA)

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

G A De Ruiter et al.
Journal of general microbiology, 139(7), 1557-1564 (1993-07-01)
A monoclonal antibody (mAb) was raised against extracellular polysaccharides from Mucor racemosus after intrasplenic immunization of mice. An indirect ELISA and a dot-blot assay were developed with this mAb. The IgG antibody was found to be very specific for all
Marta Renato et al.
Plant physiology, 166(2), 920-933 (2014-08-16)
During tomato (Solanum lycopersicum) fruit ripening, chloroplasts differentiate into photosynthetically inactive chromoplasts. It was recently reported that tomato chromoplasts can synthesize ATP through a respiratory process called chromorespiration. Here we show that chromoplast oxygen consumption is stimulated by the electron
Karina Bravo-Tello et al.
PloS one, 12(11), e0187696-e0187696 (2017-11-09)
Soybean meal has been used in many commercial diets for farm fish; despite this component inducing intestinal inflammation. On the other hand, microalgae have increasingly been used as dietary supplements in fish feed. Nevertheless, the vast quantity of microalgae species
Sacha A Jensen et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(28), 10155-10160 (2014-07-02)
Fibrillin microfibrils are 10-12 nm diameter, extracellular matrix assemblies that provide dynamic tissues of metazoan species with many of their biomechanical properties as well as sequestering growth factors and cytokines. Assembly of fibrillin monomers into microfibrils is thought to occur
Qinyi Zhu et al.
Oncology letters, 13(6), 4302-4306 (2017-06-11)
Epithelial ovarian cancer (EOC) is one of the main causes of cancer-associated mortality in females with gynecological malignancies. Duffy antigen receptor for chemokines (DARC) has previously been reported to be involved in tumor growth and the inhibition of tumor metastasis.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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