C1821

モノクロナール抗パンカドヘリン マウス宿主抗体

clone CH-19, ascites fluid

• 別名: モノクロナール抗パンカドヘリン
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: CH-19, monoclonal
• 分子量: antigen 135 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: canine, frog, snake, guinea pig, chicken, pig, feline, bovine, rabbit, hamster, Psammomys (sand rat), goat, rat, human, sheep, mouse
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested animal heart sections; indirect immunofluorescence: 1:500 using cultured MDBK cells; microarray: suitable; western blot: suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: P12830
• アプリケーション: research pathology
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CDH1(999) , CDH2(1000) , CDH3(1001) ; mouse ... Cdh1(12550) , Cdh2(12558) , Cdh3(12560) ; rat ... Cdh1(83502) , Cdh2(83501) , Cdh3(116777)

詳細

詳細

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca²⁺ -dependent cell-cell adhesion. The N-terminal part of these molecule is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually a cytoplasmic C-terminal tail which mediates interaction with the microfilament system through molecules such as catenins, plakoglobin, vinculin, and α-actinin.
Cadherins that are primarily located in areas of cell-cell contacts are involved in selective cell sorting and in the mechanical cytoplasmic response. They are implicated in morphogenetic processes, intercellular signalling and in tumor invasiveness and metastasis.
Multiple cadherins have been characterized from diverse species and tissues including E-Cadherin, N-Cadherin (A-CAM), P-Cadherin, V-Cadherin, R-Cadherin and T-Cadherin. Specific polyclonal antibodies against a highly conserved sequence from the cytoplasmic C-terminal of N-Cadherin has been prepared.

Neural-cadherin (N-cadherin) is a member of the classic cadherin family. It is mainly expressed in neural and mesenchymal tissues.

特異性

カドヘリンファミリ-の分子やC末端にカドヘリンテ-ルを含む遺伝子組換えタンパクが検出されるほか、カドヘリンの種類を問わず細胞間の接着結合が染色されます。

Monoclonal Anti-Pan Cadherin antibody reacts extensively with all known members of the cadherin family. The cadherins migrate in the molecular weight range of 125 to 140 kDa and are tissue dependent. The antibody reacts using frozen sections, protease-digested, formalin-fixed paraffin-embedded, Bouin′s-fixed and acetone-fixed tissue sections. It reacts with cultured cell line preparations. Cross-reaction has been observed with human, rabbit, bovine, goat, sheep, dog, pig, guinea pig, hamster, cat, rat, mouse, psammomys, chicken, frog and snake.

免疫原

ニワトリN-カドヘリンのC末端アミノ酸に相当する合成ペプチド(N末端リジン付加, 24アミノ酸)のKLH結合体

アプリケーション

Monoclonal Anti-Pan Cadherin antibody produced in mouse has been used in:

• fluorescence imaging
• immunohistochemistry
• western blotting
• immunoblotting
• immunofluorescence

Monoclonal Anti-Pan Cadherin may be used for: immunochemical and immunocytochemical detection of members of the cadherin family in normal and neoplastic cells and tissues; identification of genetically engineered proteins containing the C-terminal cadherin tail; screening of cDNA expression libraries for identification of novel members of the cadherin family and demonstration of adherens-type cell-cell junctions irrespective of their cadherin subtype.

生物化学的／生理学的作用

Neural-cadherin (N-cadherin) has an oncogenic role in bladder cancer, breast cancer, prostate cancer and melanoma. It stimulates the heterotypic adhesion of melanoma cells to dermal fibroblast cells. N-cadherin acts as a critical modulator for the migration of neuronal progenitors. It is essential for the proper cohesion and polarisation of neuroepithelial cells. N-cadherin is important for normal gastrulation and neural crest development. It is required for myogenesis and myotube formation.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable