F5016

モノクロナール抗ヒトIgG (Fc特異的)−FITC マウス宿主抗体

clone HP-6017, purified from hybridoma cell culture

• 別名: モノクロナール抗ヒトIgG (Fc特異的)
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: FITC conjugate
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: secondary antibodies
• クローン: HP-6017, monoclonal
• フォーム: buffered aqueous solution
• 化学種の反応性: rabbit, sheep, horse (IgG), goat, human
• 保管条件: protect from light
• テクニック: dot immunobinding: 1:16; particle immunofluorescence: 1:16
• アイソタイプ: IgG2a
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. The light comprises of one variable N-terminal region and a constant C-terminal region. The heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.

特異性

この抗体は、ヒトIgGのFc部分に特異的であり、すべてのヒトIgGサブクラスに共通のエピトープを認識します。この抗体は、IUIS/WHO研究でFc特異的試薬として採用されました。

アプリケーション

Monoclonal Anti-Human IgG (Fc specific)-FITC antibody produced in mouse has been used in:

• Fluorescent Dot Immunobinding Assay (F-DIBA)
• Particle Immunofluorescent Assay (F-IFMA)
• flow cytometry

生物化学的／生理学的作用

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), containing the H chain constant region. IgG antibody have enormous therapeutic potential and the Fc is for the development of therapeutic antibody. Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding.

物理的形状

0.01 M PBS溶液(pH 8.0, 1%非働化BSA, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H317,H334
• 注意書き: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• 危険有害性の分類: Resp. Sens. 1 - Skin Sens. 1
• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable