H9289

抗ヒストンH3 (N-末端) ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Histone H3 Detection, Histone H3 Detection Antibody, Rabbit Anti-Histone H3
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~17 kDa
• 化学種の反応性: human
• 強化検証: independent; Learn more about Antibody Enhanced Validation
• テクニック: indirect immunofluorescence: 2-4 μg/mL using A431 cells.; western blot: 0.2-0.5 μg/mL using PC12 cell lysate and A431 cell lysate.
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HIST1H3A(8350)

詳細

詳細

Anti-Histone H3 (N-terminal) specifically recognizes human histone H3 (~17kDa). Histone H3/ histone cluster 1 H3 family member a (HIST1H3A) is a core member of the histone family. HIST1H3A is encoded by the gene mapped to human chromosome 6p22.2. The encoded protein is composed of ~136 amino acid.

免疫原

ヒトヒストンH3のアミノ酸配列1~20に相当する合成ペプチドのKLH結合体。

アプリケーション

Anti-histone H3 (N-terminal) antibody produced in rabbit has been used in western blotting and native chromatin immunoprecipitation assay.

生物化学的／生理学的作用

Histones acts as structural scaffolds for DNA packaging. Histone H3 plays an important role in replication and DNA repair. Histone H3 is heavily post-translationally modified than the other members of histone family. Mutations in the gene leads to cerebellar high-grade gliomas.

物理的形状

15 mMアジ化ナトリウム含有のpH 7.4の0.01 Mリン酸緩衝食塩水溶液.

保管および安定性

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

その他情報

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)