H9773

ハイグロマイシンB Streptomyces hygroscopicus由来

lyophilized powder, suitable for plant cell culture, BioReagent

• 別名: Hygromycin
• 実験式（ヒル表記法）: C₂₀H₃₇N₃O₁₃
• CAS番号: 31282-04-9
• 分子量: 527.52
• EC Number: 250-545-5
• MDL番号: MFCD06795479
• UNSPSCコード: 12352207
• PubChem Substance ID: 329815270
• NACRES: NA.72

特性

• 製品名: ハイグロマイシンB Streptomyces hygroscopicus由来, suitable for plant cell culture, BioReagent, ≥60% (HPLC), lyophilized powder
• 由来生物: Streptomyces hygroscopicus
• 品質水準: 200
• 製品種目: BioReagent
• フォーム: lyophilized powder
• 精製方法: ion-exchange chromatography
• 濃度: ≥60% (HPLC)
• テクニック: cell culture | plant: suitable
• 色: faintly brown to brown; white to beige
• 溶解性: H₂O: soluble 50 mg/mL; ethanol: soluble; methanol: soluble
• 抗生物質活性スペクトル: fungi
• アプリケーション: agriculture
• 作用機序: protein synthesis | interferes
• 保管温度: 2-8°C
• SMILES記法: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChI Key: GRRNUXAQVGOGFE-XKIAHZFYSA-N

詳細

詳細

Chemical structure: aminoglycoside

Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

アプリケーション

ヒグロマイシンBは、分子生物学的研究において、ヒグロマイシンホスホトランスフェラーゼ(hpt)などの選択的マーカー遺伝子により形質転換される植物細胞の選択剤として用いられます。

生物化学的／生理学的作用

Hygromycin B is used in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. It is also adopted as a marker in other approaches for manipulating, introducing or deleting DNA in C. elegans. It can be inactivated by Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) through phosphorylation. The amino function of this antibiotic may be involved in a general acid-base catalysis performed by the ribozyme, acting as proton acceptors/donors.

作用機序：本製品は、原核生物におけるm-RNAテンプレートの誤読を誘発してタンパク質合成を阻害することにより作用し、翻訳を阻害する効力があります。

抗菌スペクトル：ヒグロマイシンBは、細菌、真菌およびより高等な真核細胞に対して作用します。

注意

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C.

調製ノート

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

安全性情報

• ピクトグラム: GHS06
• シグナルワード: Danger
• 危険有害性情報: H300 + H310 + H330
• 注意書き: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• 危険有害性の分類: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• 保管分類コード: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable