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製品名
デカン酸 4-ニトロフェニル, lipase substrate
品質水準
アッセイ
≥98% (TLC)
フォーム
powder
溶解性
chloroform: soluble 100 mg/mL, colorless to faintly yellow
保管温度
−20°C
SMILES記法
CCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI
1S/C16H23NO4/c1-2-3-4-5-6-7-8-9-16(18)21-15-12-10-14(11-13-15)17(19)20/h10-13H,2-9H2,1H3
InChI Key
RRNKCSIEGPOIKI-UHFFFAOYSA-N
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アプリケーション
4-Nitrophenyl decanoate has been used as a substrate for assessing the lipase activity spectrophotometrically. It has also been used as a substrate for estimating the carboxylesterases activity.
4-Nitrophenyl decanoate has been used as a substrate to assess the carboxylesterase production by recombinant Penicillium griseoroseum T55 strain. It has also been used for general esterase activity assay of the hepatopancreas extract.
生物化学的/生理学的作用
4-Nitrophenyl decanoate is a substrate for lipase enzyme. The extracellular lipase has greater hydrolysis reaction velocity with 4-nitrophenyl decanoate when compared with other 4-nitropheny esters.
適用法令
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Jan Code
N0252-BULK:
N0252-100MG:
N0252-50MG:
N0252-500MG:
N0252-VAR:
N0252-1G:
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試験成績書(COA)
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Prikladnaia biokhimiia i mikrobiologiia, 48(4), 376-382 (2012-10-06)
As a result of sequencing the genome of the termophilic alkali-tolerant lipolytic bacterium Thermosyntropha lipolytica, the gene encoding a lipase secreted into the medium was identified. The recombinant enzyme was expressed in Escherichia coli. It was isolated, purified, and functionally
Scientific reports, 8(1), 10000-10000 (2018-07-04)
We determined the effect of the His-tag on the structure, activity, stability and immobilization of LipC12, a highly active lipase from a metagenomic library. We purified LipC12 with a N-terminal His-tag and then removed the tag using tobacco etch virus
Methods in Molecular biology: Platelets and Megakaryocytes, volume 1, functional assays, 27-27 (2012)
Isolation and characterization of an enzyme from the Greenshell? mussel Perna canaliculus that hydrolyses pectenotoxins and esters of okadaic acid
Toxicon, 60(3), 406-419 (2012)
Journal of insect science (Online), 13, 77-77 (2013-11-14)
Bees of the genus Osmia are being used in crop pollination at an increasing rate. However, a short life expectancy of adult individuals limits the feasibility of their use. Cocoons of the red mason bee, Osmia rufa L. (Hymenoptera: Megachilidae)
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