NA0600

GenElute^™ HP エンドトキシンフリープラスミド メガプレップキット

1 kit sufficient for 5 preparations

• 別名: GenElute^™ HP エンドトキシンフリープラスミド メガプレップキット, Gen Elute, GenElute エンドトキシンフリー プラスミド精製キット
• UNSPSCコード: 41105501
• NACRES: NA.52

特性

• 品質水準: 200
• 使用法: kit sufficient for 5 preparations
• 環境により配慮した代替製品の特徴: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 環境により配慮した代替製品カテゴリ: , Aligned
• 保管温度: 15-25°C

詳細

詳細

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with ≤ 0.1 endotoxin units/μg can be recovered from 200 mL - 1.0 L LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digest, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

アプリケーション

高品質のエンドトキシンフリーDNAは、エンドトキシン感受性細胞を含め、制限酵素処理、ライゲーション、シークエンシング、PCR、形質転換、トランスフェクションといった用途にそのまま使用できます。

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.

特徴および利点

• Up to 5 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
• Only 90 minutes from pelleted cells to purified plasmid
• Vacuum format with no ethanol precipitation required

その他情報

詳細はwww.sigma-aldrich.com/genelutehpをご覧ください。

法的情報

GenElute is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• ピクトグラム: GHS02,GHS08,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H226,H290,H302 + H332,H315,H319,H334,H336,H372
• 注意書き: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
• ターゲットの組織: Central nervous system, Lungs
• 保管分類コード: 3 - Flammable liquids
• 引火点(°F): 77.0 °F - closed cup
• 引火点(℃): 25 °C - closed cup